Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments

TJ Rahman, R Marchant, Ibrahim Banat

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus coldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.
Original languageEnglish
Pages (from-to)209-213
JournalBiochemical Society Transactions
Volume32
Issue numberPart 2
Publication statusPublished (in print/issue) - Apr 2004

Bibliographical note

Thermophiles 2003 Meeting, Exeter, ENGLAND, SEP 15-19, 2003

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