In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus coldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.
|Journal||Biochemical Society Transactions|
|Issue number||Part 2|
|Publication status||Published - Apr 2004|
Rahman, TJ., Marchant, R., & Banat, I. (2004). Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments. Biochemical Society Transactions, 32(Part 2), 209-213.