Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments

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Abstract

In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus coldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.
LanguageEnglish
Pages209-213
JournalBiochemical Society Transactions
Volume32
Issue numberPart 2
Publication statusPublished - Apr 2004

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Geobacillus
thermophilic bacteria
edaphic factors
Bacillus thermoleovorans
Northern Ireland
ribosomal DNA
soil sampling
ribosomal RNA
new species
bacteria
genes
methodology

Cite this

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title = "Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments",
abstract = "In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50{\%}) and Geobacillus coldoxylosilyticus (34.6{\%}). Isolates F10, F20 and Tf exhibited only 93{\%} similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.",
author = "TJ Rahman and R Marchant and Ibrahim Banat",
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AU - Rahman, TJ

AU - Marchant, R

AU - Banat, Ibrahim

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PY - 2004/4

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N2 - In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus coldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.

AB - In this study, both molecular and culture-based methods were used to characterize thermophilic bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 thermophilic, aerobic, sporulating and non-sporulating bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus coldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.

M3 - Article

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SP - 209

EP - 213

JO - Biochemical Society Transactions

T2 - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

IS - Part 2

ER -