Direct DNA hybridization at disposable graphite electrodes modified with carbon nanotubes

A Erdem, P Papakonstantinou, H Murphy

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

The performance of glassy carbon ( GCE) and graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (CNTs) are compared, based on the direct electrochemical detection of nucleic acids. This is accomplished by monitoring the differential pulse voltammetry changes of the guanine signal. CNT-modified PGE compares favorably to that of the commonly used CNT-modified GCE owing to the intrinsic improved performance of the supporting PGE. The better intrinsic characteristics of the PGE are related to its composite structure and higher level of porosity compared to GCE. The performance characteristics of the direct DNA hybridization on the disposable CNT-modified PGE are studied in terms of optimum analytical conditions such as probe concentration, target concentration, hybridization time, and selectivity. The new DNA biosensor described here has shown some important advantages such being inexpensive, sensitive, selective, and able to generate reproducible results using a simple and direct electrochemical protocol.
LanguageEnglish
Pages6656-6659
JournalAnalytical Chemistry
Volume78
Issue number18
DOIs
Publication statusPublished - 15 Sep 2006

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Graphite electrodes
Carbon Nanotubes
Prostaglandins E
DNA
Multiwalled carbon nanotubes (MWCN)
Glassy carbon
Guanine
Voltammetry
Composite structures
Biosensors
Nucleic Acids
Porosity
Monitoring

Cite this

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title = "Direct DNA hybridization at disposable graphite electrodes modified with carbon nanotubes",
abstract = "The performance of glassy carbon ( GCE) and graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (CNTs) are compared, based on the direct electrochemical detection of nucleic acids. This is accomplished by monitoring the differential pulse voltammetry changes of the guanine signal. CNT-modified PGE compares favorably to that of the commonly used CNT-modified GCE owing to the intrinsic improved performance of the supporting PGE. The better intrinsic characteristics of the PGE are related to its composite structure and higher level of porosity compared to GCE. The performance characteristics of the direct DNA hybridization on the disposable CNT-modified PGE are studied in terms of optimum analytical conditions such as probe concentration, target concentration, hybridization time, and selectivity. The new DNA biosensor described here has shown some important advantages such being inexpensive, sensitive, selective, and able to generate reproducible results using a simple and direct electrochemical protocol.",
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Direct DNA hybridization at disposable graphite electrodes modified with carbon nanotubes. / Erdem, A; Papakonstantinou, P; Murphy, H.

In: Analytical Chemistry, Vol. 78, No. 18, 15.09.2006, p. 6656-6659.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Erdem, A

AU - Papakonstantinou, P

AU - Murphy, H

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