Dimensions of a projection column and architecture of VPM and POm axons in rat vibrissal cortex.

Verena C Wimmer, Randy M Bruno, Christiaan P J Kock, Thomas Kuner, Bert Sakmann

    Research output: Contribution to journalArticle

    117 Citations (Scopus)

    Abstract

    This is the first article in a series of 3 studies that investigate the anatomical determinants of thalamocortical (TC) input to excitatory neurons in a cortical column of rat primary somatosensory cortex (S1). S1 receives 2 major types of TC inputs, lemiscal and paralemniscal. Lemiscal axons arise from the ventral posteromedial nucleus (VPM) of the thalamus, whereas paralemniscal fibers originate in the posteromedial nucleus (POm). While these 2 TC projections are largely complementary in L4, overlap in other cortical layers is still a matter of debate. VPM and POm axons were specifically labeled in the same rat by virus-mediated expression of different fluorescent proteins. We show that columnar and septal projection patterns are maintained throughout most of the cortical depth with a lower degree of separation in infragranular layers, where TC axons form bands along rows. Finally, we present anatomical dimensions of "TC projection domains" for a standard column in S1.
    LanguageEnglish
    Pages2265-2276
    JournalCerebral Cortex
    Volume20
    Issue number10
    DOIs
    Publication statusPublished - Oct 2010

    Fingerprint

    Ventral Thalamic Nuclei
    Axons
    Parvovirus
    Somatosensory Cortex
    Neurons
    Proteins

    Keywords

    • Analysis of Variance
    • Animals
    • Newborn
    • Axons
    • physiology/ultrastructure
    • Cell Count
    • methods
    • Dependovirus
    • physiology
    • Green Fluorescent
    • Proteins
    • genetics
    • Luminescent Proteins
    • Microscopy
    • Confocal
    • Neural Pathways
    • Posterior Thalamic Nuclei
    • cytology
    • Presynaptic Terminals
    • Rats
    • Sensory Receptor
    • Cells
    • Somatosensory Cortex
    • anatomy /&amp
    • / histology
    • Ventral
    • Thalamic Nuclei
    • Vibrissae
    • innervation

    Cite this

    Wimmer, V. C., Bruno, R. M., Kock, C. P. J., Kuner, T., & Sakmann, B. (2010). Dimensions of a projection column and architecture of VPM and POm axons in rat vibrissal cortex. Cerebral Cortex, 20(10), 2265-2276. https://doi.org/10.1093/cercor/bhq068
    Wimmer, Verena C ; Bruno, Randy M ; Kock, Christiaan P J ; Kuner, Thomas ; Sakmann, Bert. / Dimensions of a projection column and architecture of VPM and POm axons in rat vibrissal cortex. In: Cerebral Cortex. 2010 ; Vol. 20, No. 10. pp. 2265-2276.
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    abstract = "This is the first article in a series of 3 studies that investigate the anatomical determinants of thalamocortical (TC) input to excitatory neurons in a cortical column of rat primary somatosensory cortex (S1). S1 receives 2 major types of TC inputs, lemiscal and paralemniscal. Lemiscal axons arise from the ventral posteromedial nucleus (VPM) of the thalamus, whereas paralemniscal fibers originate in the posteromedial nucleus (POm). While these 2 TC projections are largely complementary in L4, overlap in other cortical layers is still a matter of debate. VPM and POm axons were specifically labeled in the same rat by virus-mediated expression of different fluorescent proteins. We show that columnar and septal projection patterns are maintained throughout most of the cortical depth with a lower degree of separation in infragranular layers, where TC axons form bands along rows. Finally, we present anatomical dimensions of {"}TC projection domains{"} for a standard column in S1.",
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    Wimmer, VC, Bruno, RM, Kock, CPJ, Kuner, T & Sakmann, B 2010, 'Dimensions of a projection column and architecture of VPM and POm axons in rat vibrissal cortex.', Cerebral Cortex, vol. 20, no. 10, pp. 2265-2276. https://doi.org/10.1093/cercor/bhq068

    Dimensions of a projection column and architecture of VPM and POm axons in rat vibrissal cortex. / Wimmer, Verena C; Bruno, Randy M; Kock, Christiaan P J; Kuner, Thomas; Sakmann, Bert.

    In: Cerebral Cortex, Vol. 20, No. 10, 10.2010, p. 2265-2276.

    Research output: Contribution to journalArticle

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    AU - Wimmer, Verena C

    AU - Bruno, Randy M

    AU - Kock, Christiaan P J

    AU - Kuner, Thomas

    AU - Sakmann, Bert

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    N2 - This is the first article in a series of 3 studies that investigate the anatomical determinants of thalamocortical (TC) input to excitatory neurons in a cortical column of rat primary somatosensory cortex (S1). S1 receives 2 major types of TC inputs, lemiscal and paralemniscal. Lemiscal axons arise from the ventral posteromedial nucleus (VPM) of the thalamus, whereas paralemniscal fibers originate in the posteromedial nucleus (POm). While these 2 TC projections are largely complementary in L4, overlap in other cortical layers is still a matter of debate. VPM and POm axons were specifically labeled in the same rat by virus-mediated expression of different fluorescent proteins. We show that columnar and septal projection patterns are maintained throughout most of the cortical depth with a lower degree of separation in infragranular layers, where TC axons form bands along rows. Finally, we present anatomical dimensions of "TC projection domains" for a standard column in S1.

    AB - This is the first article in a series of 3 studies that investigate the anatomical determinants of thalamocortical (TC) input to excitatory neurons in a cortical column of rat primary somatosensory cortex (S1). S1 receives 2 major types of TC inputs, lemiscal and paralemniscal. Lemiscal axons arise from the ventral posteromedial nucleus (VPM) of the thalamus, whereas paralemniscal fibers originate in the posteromedial nucleus (POm). While these 2 TC projections are largely complementary in L4, overlap in other cortical layers is still a matter of debate. VPM and POm axons were specifically labeled in the same rat by virus-mediated expression of different fluorescent proteins. We show that columnar and septal projection patterns are maintained throughout most of the cortical depth with a lower degree of separation in infragranular layers, where TC axons form bands along rows. Finally, we present anatomical dimensions of "TC projection domains" for a standard column in S1.

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    KW - Cell Count

    KW - methods

    KW - Dependovirus

    KW - physiology

    KW - Green Fluorescent

    KW - Proteins

    KW - genetics

    KW - Luminescent Proteins

    KW - Microscopy

    KW - Confocal

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    KW - Posterior Thalamic Nuclei

    KW - cytology

    KW - Presynaptic Terminals

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    KW - / histology

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