DIHYDROFOLATE-REDUCTASE SYNTHESIS IN CONTINUOUS-CULTURE USING A METHOTREXATE-RESISTANT ESCHERICHIA-COLI

Research output: Contribution to journalArticle

Abstract

A methotrexate-resistant strain of Escherichia coli Type I produced exceptionally high levels of the enzyme dihydrofolate reductase (EC 1.5.1.3) in 6 h of batch fermentation. The culture had markedly improved performance under chemostat culture conditions in terms of enzyme yield and output. Temperature, pH, dilution rates, and nutrient composition were optimized under chemostat culture conditions. A maximum enzyme yield of 10,360 U l-1 and a specific activity of 22.84 U mg-1 were obtained under chemostat conditions at PH 7.0, temperature 37-degrees-C, and dilution rate 0.2 h-1, using media containing 1.0 and 0.6% (w/v) dextrose and yeast extract, respectively. The culture's performance and enzyme yields in chemostat and the feasibility of large-scale production are discussed.
LanguageEnglish
Pages652-656
JournalEnzyme and Microbial Technology
Volume15
Issue number8
Publication statusPublished - Aug 1993

Fingerprint

dihydrofolate reductase
methotrexate
Escherichia coli
synthesis
enzymes
batch fermentation
yeast extract
temperature
nutrient content
glucose

Cite this

@article{8dc3a9f54bd6400699a2bebef949937c,
title = "DIHYDROFOLATE-REDUCTASE SYNTHESIS IN CONTINUOUS-CULTURE USING A METHOTREXATE-RESISTANT ESCHERICHIA-COLI",
abstract = "A methotrexate-resistant strain of Escherichia coli Type I produced exceptionally high levels of the enzyme dihydrofolate reductase (EC 1.5.1.3) in 6 h of batch fermentation. The culture had markedly improved performance under chemostat culture conditions in terms of enzyme yield and output. Temperature, pH, dilution rates, and nutrient composition were optimized under chemostat culture conditions. A maximum enzyme yield of 10,360 U l-1 and a specific activity of 22.84 U mg-1 were obtained under chemostat conditions at PH 7.0, temperature 37-degrees-C, and dilution rate 0.2 h-1, using media containing 1.0 and 0.6{\%} (w/v) dextrose and yeast extract, respectively. The culture's performance and enzyme yields in chemostat and the feasibility of large-scale production are discussed.",
author = "{Singh - Nee Nigam}, Poonam and Ibrahim Banat and BA Kelly and R Marchant",
year = "1993",
month = "8",
language = "English",
volume = "15",
pages = "652--656",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier",
number = "8",

}

TY - JOUR

T1 - DIHYDROFOLATE-REDUCTASE SYNTHESIS IN CONTINUOUS-CULTURE USING A METHOTREXATE-RESISTANT ESCHERICHIA-COLI

AU - Singh - Nee Nigam, Poonam

AU - Banat, Ibrahim

AU - Kelly, BA

AU - Marchant, R

PY - 1993/8

Y1 - 1993/8

N2 - A methotrexate-resistant strain of Escherichia coli Type I produced exceptionally high levels of the enzyme dihydrofolate reductase (EC 1.5.1.3) in 6 h of batch fermentation. The culture had markedly improved performance under chemostat culture conditions in terms of enzyme yield and output. Temperature, pH, dilution rates, and nutrient composition were optimized under chemostat culture conditions. A maximum enzyme yield of 10,360 U l-1 and a specific activity of 22.84 U mg-1 were obtained under chemostat conditions at PH 7.0, temperature 37-degrees-C, and dilution rate 0.2 h-1, using media containing 1.0 and 0.6% (w/v) dextrose and yeast extract, respectively. The culture's performance and enzyme yields in chemostat and the feasibility of large-scale production are discussed.

AB - A methotrexate-resistant strain of Escherichia coli Type I produced exceptionally high levels of the enzyme dihydrofolate reductase (EC 1.5.1.3) in 6 h of batch fermentation. The culture had markedly improved performance under chemostat culture conditions in terms of enzyme yield and output. Temperature, pH, dilution rates, and nutrient composition were optimized under chemostat culture conditions. A maximum enzyme yield of 10,360 U l-1 and a specific activity of 22.84 U mg-1 were obtained under chemostat conditions at PH 7.0, temperature 37-degrees-C, and dilution rate 0.2 h-1, using media containing 1.0 and 0.6% (w/v) dextrose and yeast extract, respectively. The culture's performance and enzyme yields in chemostat and the feasibility of large-scale production are discussed.

M3 - Article

VL - 15

SP - 652

EP - 656

JO - Enzyme and Microbial Technology

T2 - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 8

ER -