Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool

David Gibson, David Bramwell, Catriona Scaife

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    Abstract

    Difference in-gel electrophoresis (DIGE) is a recent adaptation of conventional two-dimensional gel electrophoresis (2-DE) that incorporates novel fluorescent labels, has multiplex attributes, and boasts software-assisted image analysis. Combined, these characteristics offer significant benefits in accuracy and reproducibility to quantify differential protein expression levels between biological samples. The DIGE technique and materials required to perform it are described in detail within. The principles behind consistent gel image acquisition and reliable image analysis are also considered. Within the context of biomarker and drug target discovery, this method simplifies analysis, increases sample throughput, and represents a reliable 2-DE platform.
    Original languageEnglish
    Title of host publicationBiomarker Methods in Drug Discovery and Development
    Place of PublicationUSA
    Pages189-209
    DOIs
    Publication statusPublished - 2008

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    Keywords

    • two dimensional gel electrophoresis
    • isoelectric focusing
    • difference in-gel electrophoresis
    • Cyanine dyes
    • proteomics
    • bioinformatics

    Cite this

    Gibson, D., Bramwell, D., & Scaife, C. (2008). Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool. In Biomarker Methods in Drug Discovery and Development (pp. 189-209). USA. https://doi.org/10.1007/978-1-59745-463-6_9