Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool

David Gibson; David Bramwell; Catriona Scaife

doi:10.1007/978-1-59745-463-6_9

Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool

David Gibson, David Bramwell, Catriona Scaife

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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Abstract

Difference in-gel electrophoresis (DIGE) is a recent adaptation of conventional two-dimensional gel electrophoresis (2-DE) that incorporates novel fluorescent labels, has multiplex attributes, and boasts software-assisted image analysis. Combined, these characteristics offer significant benefits in accuracy and reproducibility to quantify differential protein expression levels between biological samples. The DIGE technique and materials required to perform it are described in detail within. The principles behind consistent gel image acquisition and reliable image analysis are also considered. Within the context of biomarker and drug target discovery, this method simplifies analysis, increases sample throughput, and represents a reliable 2-DE platform.

Original language	English
Title of host publication	Biomarker Methods in Drug Discovery and Development
Place of Publication	USA
Publisher	Humana Press
Pages	189-209
ISBN (Print)	978-1-934115-23-7
DOIs	https://doi.org/10.1007/978-1-59745-463-6_9
Publication status	Published - 2008

Keywords

two dimensional gel electrophoresis
isoelectric focusing
difference in-gel electrophoresis
Cyanine dyes
proteomics
bioinformatics

Access to Document

10.1007/978-1-59745-463-6_9

DIGE Book Wang 9781597454636 Chap09 311207Accepted author manuscript, 3.74 MB

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AB - Difference in-gel electrophoresis (DIGE) is a recent adaptation of conventional two-dimensional gel electrophoresis (2-DE) that incorporates novel fluorescent labels, has multiplex attributes, and boasts software-assisted image analysis. Combined, these characteristics offer significant benefits in accuracy and reproducibility to quantify differential protein expression levels between biological samples. The DIGE technique and materials required to perform it are described in detail within. The principles behind consistent gel image acquisition and reliable image analysis are also considered. Within the context of biomarker and drug target discovery, this method simplifies analysis, increases sample throughput, and represents a reliable 2-DE platform.

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KW - isoelectric focusing

KW - difference in-gel electrophoresis

KW - Cyanine dyes

KW - proteomics

KW - bioinformatics

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