Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers

RW Welch, E Turley, SF Sweetman, G Kennedy, AR Collins, A Dunne, MBE Livingstone, PG McKenna, VJ McKelvey-Martin, JJ Strain

Research output: Contribution to journalArticle

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Abstract

Deficiencies of antioxidant nutrients have been implicated in the etiology of lung and other cancers. However, most intervention trials with antioxidant nutrients have not shown beneficial effects, and some have indicated that beta-carotene may be deleterious. This randomized, double-blind, placebo-controlled study evaluated the effects of five short-term (4-wk) antioxidant nutrient supplement regimens [ascorbic acid (350 mg), RRR-alpha-tocopherol (250 mg), beta-carotene (60 mg), selenium (80 mu g as sodium selenite), ascorbic acid (350 mg) + RRR-alpha-tocopherol (250 mg)] on plasma antioxidants and mononuclear leukocyte DNA damage in male smokers (n = 9) and nonsmokers (n = 12). Plasma concentrations of ascorbic acid and tocopherol were significantly increased by supplementation, but there was no significant change in plasma beta-carotene or blood glutathione peroxidase activity after supplementation with beta-carotene or selenium. DNA damage in mononuclear leukocytes, as assessed by comet assay, was not affected by any supplementation regimen. DNA damage, as assessed by 8-hydroxydeoxyguanosine in mononuclear leukocytes, was nor influenced by ascorbic acid, alpha-tocopherol, or selenium supplementation in smokers or nonsmokers, but beta-carotene supplementation resulted in significant differences between smokers and nonsmokers in the level of oxidative DNA damage, with decreases in smokers and increases in smokers. This is a further indication of the differential effects of supplemental beta-carotene in smokers and nonsmokers.
LanguageEnglish
Pages167-172
JournalNUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
Volume34
Issue number2
Publication statusPublished - 1999

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DNA damage
beta-carotene
antioxidants
mononuclear leukocytes
ascorbic acid
alpha-tocopherol
selenium
sodium selenite
nutrients
tocopherols
glutathione peroxidase
placebos
etiology
dietary supplements
lungs
neoplasms
blood
assays

Cite this

Welch, RW., Turley, E., Sweetman, SF., Kennedy, G., Collins, AR., Dunne, A., ... Strain, JJ. (1999). Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 34(2), 167-172.
Welch, RW ; Turley, E ; Sweetman, SF ; Kennedy, G ; Collins, AR ; Dunne, A ; Livingstone, MBE ; McKenna, PG ; McKelvey-Martin, VJ ; Strain, JJ. / Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers. In: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL. 1999 ; Vol. 34, No. 2. pp. 167-172.
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abstract = "Deficiencies of antioxidant nutrients have been implicated in the etiology of lung and other cancers. However, most intervention trials with antioxidant nutrients have not shown beneficial effects, and some have indicated that beta-carotene may be deleterious. This randomized, double-blind, placebo-controlled study evaluated the effects of five short-term (4-wk) antioxidant nutrient supplement regimens [ascorbic acid (350 mg), RRR-alpha-tocopherol (250 mg), beta-carotene (60 mg), selenium (80 mu g as sodium selenite), ascorbic acid (350 mg) + RRR-alpha-tocopherol (250 mg)] on plasma antioxidants and mononuclear leukocyte DNA damage in male smokers (n = 9) and nonsmokers (n = 12). Plasma concentrations of ascorbic acid and tocopherol were significantly increased by supplementation, but there was no significant change in plasma beta-carotene or blood glutathione peroxidase activity after supplementation with beta-carotene or selenium. DNA damage in mononuclear leukocytes, as assessed by comet assay, was not affected by any supplementation regimen. DNA damage, as assessed by 8-hydroxydeoxyguanosine in mononuclear leukocytes, was nor influenced by ascorbic acid, alpha-tocopherol, or selenium supplementation in smokers or nonsmokers, but beta-carotene supplementation resulted in significant differences between smokers and nonsmokers in the level of oxidative DNA damage, with decreases in smokers and increases in smokers. This is a further indication of the differential effects of supplemental beta-carotene in smokers and nonsmokers.",
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Welch, RW, Turley, E, Sweetman, SF, Kennedy, G, Collins, AR, Dunne, A, Livingstone, MBE, McKenna, PG, McKelvey-Martin, VJ & Strain, JJ 1999, 'Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers', NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, vol. 34, no. 2, pp. 167-172.

Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers. / Welch, RW; Turley, E; Sweetman, SF; Kennedy, G; Collins, AR; Dunne, A; Livingstone, MBE; McKenna, PG; McKelvey-Martin, VJ; Strain, JJ.

In: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, Vol. 34, No. 2, 1999, p. 167-172.

Research output: Contribution to journalArticle

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T1 - Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers

AU - Welch, RW

AU - Turley, E

AU - Sweetman, SF

AU - Kennedy, G

AU - Collins, AR

AU - Dunne, A

AU - Livingstone, MBE

AU - McKenna, PG

AU - McKelvey-Martin, VJ

AU - Strain, JJ

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N2 - Deficiencies of antioxidant nutrients have been implicated in the etiology of lung and other cancers. However, most intervention trials with antioxidant nutrients have not shown beneficial effects, and some have indicated that beta-carotene may be deleterious. This randomized, double-blind, placebo-controlled study evaluated the effects of five short-term (4-wk) antioxidant nutrient supplement regimens [ascorbic acid (350 mg), RRR-alpha-tocopherol (250 mg), beta-carotene (60 mg), selenium (80 mu g as sodium selenite), ascorbic acid (350 mg) + RRR-alpha-tocopherol (250 mg)] on plasma antioxidants and mononuclear leukocyte DNA damage in male smokers (n = 9) and nonsmokers (n = 12). Plasma concentrations of ascorbic acid and tocopherol were significantly increased by supplementation, but there was no significant change in plasma beta-carotene or blood glutathione peroxidase activity after supplementation with beta-carotene or selenium. DNA damage in mononuclear leukocytes, as assessed by comet assay, was not affected by any supplementation regimen. DNA damage, as assessed by 8-hydroxydeoxyguanosine in mononuclear leukocytes, was nor influenced by ascorbic acid, alpha-tocopherol, or selenium supplementation in smokers or nonsmokers, but beta-carotene supplementation resulted in significant differences between smokers and nonsmokers in the level of oxidative DNA damage, with decreases in smokers and increases in smokers. This is a further indication of the differential effects of supplemental beta-carotene in smokers and nonsmokers.

AB - Deficiencies of antioxidant nutrients have been implicated in the etiology of lung and other cancers. However, most intervention trials with antioxidant nutrients have not shown beneficial effects, and some have indicated that beta-carotene may be deleterious. This randomized, double-blind, placebo-controlled study evaluated the effects of five short-term (4-wk) antioxidant nutrient supplement regimens [ascorbic acid (350 mg), RRR-alpha-tocopherol (250 mg), beta-carotene (60 mg), selenium (80 mu g as sodium selenite), ascorbic acid (350 mg) + RRR-alpha-tocopherol (250 mg)] on plasma antioxidants and mononuclear leukocyte DNA damage in male smokers (n = 9) and nonsmokers (n = 12). Plasma concentrations of ascorbic acid and tocopherol were significantly increased by supplementation, but there was no significant change in plasma beta-carotene or blood glutathione peroxidase activity after supplementation with beta-carotene or selenium. DNA damage in mononuclear leukocytes, as assessed by comet assay, was not affected by any supplementation regimen. DNA damage, as assessed by 8-hydroxydeoxyguanosine in mononuclear leukocytes, was nor influenced by ascorbic acid, alpha-tocopherol, or selenium supplementation in smokers or nonsmokers, but beta-carotene supplementation resulted in significant differences between smokers and nonsmokers in the level of oxidative DNA damage, with decreases in smokers and increases in smokers. This is a further indication of the differential effects of supplemental beta-carotene in smokers and nonsmokers.

M3 - Article

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EP - 172

JO - Nutrition and Cancer - An International Journal

T2 - Nutrition and Cancer - An International Journal

JF - Nutrition and Cancer - An International Journal

SN - 0163-5581

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ER -

Welch RW, Turley E, Sweetman SF, Kennedy G, Collins AR, Dunne A et al. Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL. 1999;34(2):167-172.