Development of liposome gel based formulations for intravaginal deliveryof the recombinant HIV-1 envelope protein CN54gp140

Prem N Gupta, Aditya Pattani, Rhonda M Curran, Vicky L Kett, Gavin P Andrews, Ryan J Morrow, A David Woolfson, R Karl Malcolm

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Mucosally-administered vaccine strategies are widely investigated as a promising means of preventing HIV infection. This study describes the development of liposomal gel formulations, and novel lyophilised variants, comprising HIV-1 envelope glycoprotein, CN54gp140, encapsulated within neutral, positively charged or negatively charged liposomes. The CN54gp140 liposomes were evaluated for mean vesicle diameter, polydispersity, morphology, zeta potential and antigen encapsulation efficiency before being incorporated into hydroxyethyl cellulose (HEC) aqueous gel and subsequently lyophilised to produce a rod-shaped solid dosage form for practical vaginal application. The lyophilised liposome–HEC rods were evaluated for moisture content and redispersibility in simulated vaginal fluid. Since these rods are designed to revert to gel form following intravaginal application, mucoadhesive, mechanical (compressibility and hardness) and rheological properties of the reformed gels were evaluated. The liposomes exhibited good encapsulation efficiency and the gels demonstrated suitable mucoadhesive strength. The freezedried liposome–HEC formulations represent a novel formulation strategy that could offer potential as stable and practical dosage form.
    LanguageEnglish
    Pages315-322
    JournalEuropean Journal of Pharmaceutical Sciences
    Volume46
    Issue number5
    DOIs
    Publication statusPublished - 14 Feb 2012

    Fingerprint

    Liposomes
    HIV-1
    Gels
    Cellulose
    Dosage Forms
    Proteins
    Hardness
    HIV Infections
    Glycoproteins
    Vaccines
    Antigens

    Cite this

    Gupta, Prem N ; Pattani, Aditya ; Curran, Rhonda M ; Kett, Vicky L ; Andrews, Gavin P ; Morrow, Ryan J ; Woolfson, A David ; Malcolm, R Karl. / Development of liposome gel based formulations for intravaginal deliveryof the recombinant HIV-1 envelope protein CN54gp140. In: European Journal of Pharmaceutical Sciences. 2012 ; Vol. 46, No. 5. pp. 315-322.
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    Development of liposome gel based formulations for intravaginal deliveryof the recombinant HIV-1 envelope protein CN54gp140. / Gupta, Prem N; Pattani, Aditya; Curran, Rhonda M; Kett, Vicky L; Andrews, Gavin P; Morrow, Ryan J; Woolfson, A David; Malcolm, R Karl.

    In: European Journal of Pharmaceutical Sciences, Vol. 46, No. 5, 14.02.2012, p. 315-322.

    Research output: Contribution to journalArticle

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    AU - Andrews, Gavin P

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    AU - Woolfson, A David

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