Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1

Prem N Gupta; Aditya Pattani; R Karl Malcolm; Rhonda M Curran; Gavin P Andrews

doi:10.1016/j.jconrel.2010.07.080

Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1

Prem N Gupta, Aditya Pattani, R Karl Malcolm, Rhonda M Curran, Gavin P Andrews

Research output: Contribution to journal › Article › peer-review

Abstract

The present investigation dealswith development and characterization of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods,which were then characterized for moisture content. The redispersibility of the liposomes-based freezedried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.

Original language	English
Pages (from-to)	e108-e110
Journal	Journal of Controlled Release
Volume	148
DOIs	https://doi.org/10.1016/j.jconrel.2010.07.080
Publication status	Published (in print/issue) - 2010

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10.1016/j.jconrel.2010.07.080

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title = "Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1",

abstract = "The present investigation dealswith development and characterization of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods,which were then characterized for moisture content. The redispersibility of the liposomes-based freezedried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.",

author = "Gupta, {Prem N} and Aditya Pattani and Malcolm, {R Karl} and Curran, {Rhonda M} and Andrews, {Gavin P}",

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T1 - Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1

AU - Gupta, Prem N

AU - Pattani, Aditya

AU - Malcolm, R Karl

AU - Curran, Rhonda M

AU - Andrews, Gavin P

PY - 2010

Y1 - 2010

N2 - The present investigation dealswith development and characterization of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods,which were then characterized for moisture content. The redispersibility of the liposomes-based freezedried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.

AB - The present investigation dealswith development and characterization of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods,which were then characterized for moisture content. The redispersibility of the liposomes-based freezedried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.

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DO - 10.1016/j.jconrel.2010.07.080

M3 - Article

SN - 1873-4995

VL - 148

SP - e108-e110

JO - Journal of Controlled Release

JF - Journal of Controlled Release

ER -

Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1

Abstract

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