Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1

Prem N Gupta, Aditya Pattani, R Karl Malcolm, Rhonda M Curran, Gavin P Andrews

    Research output: Contribution to journalArticle

    Abstract

    The present investigation dealswith development and characterization of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods,which were then characterized for moisture content. The redispersibility of the liposomes-based freezedried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.
    LanguageEnglish
    Pagese108-e110
    JournalJournal of Controlled Release
    Volume148
    DOIs
    Publication statusPublished - 2010

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    Liposomes
    HIV-1
    Antigens
    Gels
    Dosage Forms
    HIV Infections
    Immunization
    Polymers
    Vaccines

    Cite this

    Gupta, Prem N ; Pattani, Aditya ; Malcolm, R Karl ; Curran, Rhonda M ; Andrews, Gavin P. / Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1. In: Journal of Controlled Release. 2010 ; Vol. 148. pp. e108-e110.
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    abstract = "The present investigation dealswith development and characterization of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods,which were then characterized for moisture content. The redispersibility of the liposomes-based freezedried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection.",
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    Development of liposome-based freeze-dried rods for vaginalvaccine delivery against HIV-1. / Gupta, Prem N; Pattani, Aditya; Malcolm, R Karl; Curran, Rhonda M; Andrews, Gavin P.

    In: Journal of Controlled Release, Vol. 148, 2010, p. e108-e110.

    Research output: Contribution to journalArticle

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    AU - Andrews, Gavin P

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