Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function

Li Fang Zhu; Yuanfa Yao; Zeeshan Ahmad; Ming Wei Chang

doi:10.1016/j.procbio.2019.05.025

Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function

Li Fang Zhu
, Yuanfa Yao
, Zeeshan Ahmad
, Ming Wei Chang

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

351 Downloads (Pure)

Abstract

A new natural proteoglycan from Ganoderma lucidum spore powder (GLSP) was obtained via solvent extraction and optimized using response surface methodology (RSM). The differences among the characteristics of the new proteoglycan from cracked (proteoglycan-C) and uncracked GLSP (proteoglycan-UC) were explored. The SDS-PAGE results showed the molecular weight of protein contained in proteoglycan-UC (55, 72, 95 kDa) was different to that proteoglycan-C (43, 95 kDa). The differences of these amino acids (Glu, Arg, Leu and Lys) content and monosaccharides (Fuc, Gal, Glc, Man and Gal-AC) content between proteoglycan-C and proteoglycan-UC were determined by HPLC/ ion chromatography. The 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of proteoglycan-C were higher than that proteoglycan-UC. The proteoglycan-UC exhibited stronger hypoglycemic and antibacterial effects against E. coli and S. aureus, and the proteoglycan-C and proteoglycan-UC showed antitumor effects with potential for therapeutic applications.

Original language	English
Pages (from-to)	103-111
Number of pages	9
Journal	Process Biochemistry
Volume	84
Early online date	23 May 2019
DOIs	https://doi.org/10.1016/j.procbio.2019.05.025
Publication status	Published (in print/issue) - 1 Sept 2019

Funding

This research was financially supported by the National Nature Science Foundation of China (No. 81771960 ), the Fundamental Research Funds for the Central Universities ( 2017QNA5017 ), the Major Scientific Project of Zhejiang Lab (No. 2018DG0ZX01 ) and Key Technologies R&D Program of Zhejiang Province ( 2015C02035 ). Appendix A

Keywords

Antibacterial
Antitumor
Ganoderma lucidum spore powder
Hypoglycemic
Proteoglycan

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BP_Revised ManuscriptAuthor Accepted Manuscript, 3.83 MBLicence: CC BY-NC-ND

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title = "Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function",

abstract = "A new natural proteoglycan from Ganoderma lucidum spore powder (GLSP) was obtained via solvent extraction and optimized using response surface methodology (RSM). The differences among the characteristics of the new proteoglycan from cracked (proteoglycan-C) and uncracked GLSP (proteoglycan-UC) were explored. The SDS-PAGE results showed the molecular weight of protein contained in proteoglycan-UC (55, 72, 95 kDa) was different to that proteoglycan-C (43, 95 kDa). The differences of these amino acids (Glu, Arg, Leu and Lys) content and monosaccharides (Fuc, Gal, Glc, Man and Gal-AC) content between proteoglycan-C and proteoglycan-UC were determined by HPLC/ ion chromatography. The 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of proteoglycan-C were higher than that proteoglycan-UC. The proteoglycan-UC exhibited stronger hypoglycemic and antibacterial effects against E. coli and S. aureus, and the proteoglycan-C and proteoglycan-UC showed antitumor effects with potential for therapeutic applications.",

keywords = "Antibacterial, Antitumor, Ganoderma lucidum spore powder, Hypoglycemic, Proteoglycan",

author = "Zhu, \{Li Fang\} and Yuanfa Yao and Zeeshan Ahmad and Chang, \{Ming Wei\}",

year = "2019",

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doi = "10.1016/j.procbio.2019.05.025",

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T1 - Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function

AU - Zhu, Li Fang

AU - Yao, Yuanfa

AU - Ahmad, Zeeshan

AU - Chang, Ming Wei

PY - 2019/9/1

Y1 - 2019/9/1

N2 - A new natural proteoglycan from Ganoderma lucidum spore powder (GLSP) was obtained via solvent extraction and optimized using response surface methodology (RSM). The differences among the characteristics of the new proteoglycan from cracked (proteoglycan-C) and uncracked GLSP (proteoglycan-UC) were explored. The SDS-PAGE results showed the molecular weight of protein contained in proteoglycan-UC (55, 72, 95 kDa) was different to that proteoglycan-C (43, 95 kDa). The differences of these amino acids (Glu, Arg, Leu and Lys) content and monosaccharides (Fuc, Gal, Glc, Man and Gal-AC) content between proteoglycan-C and proteoglycan-UC were determined by HPLC/ ion chromatography. The 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of proteoglycan-C were higher than that proteoglycan-UC. The proteoglycan-UC exhibited stronger hypoglycemic and antibacterial effects against E. coli and S. aureus, and the proteoglycan-C and proteoglycan-UC showed antitumor effects with potential for therapeutic applications.

AB - A new natural proteoglycan from Ganoderma lucidum spore powder (GLSP) was obtained via solvent extraction and optimized using response surface methodology (RSM). The differences among the characteristics of the new proteoglycan from cracked (proteoglycan-C) and uncracked GLSP (proteoglycan-UC) were explored. The SDS-PAGE results showed the molecular weight of protein contained in proteoglycan-UC (55, 72, 95 kDa) was different to that proteoglycan-C (43, 95 kDa). The differences of these amino acids (Glu, Arg, Leu and Lys) content and monosaccharides (Fuc, Gal, Glc, Man and Gal-AC) content between proteoglycan-C and proteoglycan-UC were determined by HPLC/ ion chromatography. The 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of proteoglycan-C were higher than that proteoglycan-UC. The proteoglycan-UC exhibited stronger hypoglycemic and antibacterial effects against E. coli and S. aureus, and the proteoglycan-C and proteoglycan-UC showed antitumor effects with potential for therapeutic applications.

KW - Antibacterial

KW - Antitumor

KW - Ganoderma lucidum spore powder

KW - Hypoglycemic

KW - Proteoglycan

UR - https://www.scopus.com/pages/publications/85066097224

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DO - 10.1016/j.procbio.2019.05.025

M3 - Article

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SN - 1359-5113

VL - 84

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EP - 111

JO - Process Biochemistry

JF - Process Biochemistry

ER -

Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function

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