Development of a specific radioimmunoassay for vitamin B12 and its application in the diagnosis of cobalt deficiency in sheep

D. G. Kennedy, F. P.M. O'Harte, W. J. Blanchflower, D. A. Rice

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8 Citations (Scopus)

Abstract

A radioimmunoassay (RIA) for vitamin B12 is described. Antisera were raised in rabbits using a conjugate between 5′-O-succinyl cyanocobalamin and chicken serum albumin. The antibody is largely specific for the β (upper) face of the vitamin B12 molecule. The RIA can detect vitamin B12 down to concentrations of 37 pmol/L and correlated well (r=0.980, p<0.001) with a commercial radioassay. The use of this RIA, together with an assay for plasma methylmalonic acid, in the diagnosis of cobalt/vitamin B12 deficiency in sheep is described.

Original languageEnglish
Pages (from-to)255-265
Number of pages11
JournalVeterinary Research Communications
Volume14
Issue number4
DOIs
Publication statusPublished - Jul 1990

Keywords

  • cobalt
  • diagnosis
  • radioimmunoassay
  • sheep
  • vitamin B

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