Development of a novel HPTLC fingerprint method for simultaneous estimation of berberine and rutin in medicinal plants and their pharmaceutical preparations followed by its application in antioxidant assay

Saurabh Satija, Murtaza M. Tambuwala, Kavita Pabreja, Hamid A. Bakshi, Dinesh Kumar Chellappan, Alaa A. Aljabali, Srinivas Nammi, Thakur Gurjeet Singh, Harish Dureja, Gaurav Gupta, Kamal Dua, Meenu Mehta, Munish Garg

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Abstract

The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile phase. Detection was completed densitometrically at 254 nm. The R F estimation of berberine and rutin was observed to be 0.67 ± 0.02 and 0.47 ± 0.02, respectively. The developed HPTLC method was validated according to ICH guidelines; the method was specific, linear and accurate and can be used to determine berberine and rutin in marketed herbal preparations. The Tinospora cordifolia plant extract was further evaluated for antioxidant activity using HPTLC, and berberine was found to be more active than rutin during DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. The method was found simple, rapid, accurate, specific and robust for the analysis of berberine and rutin in crude drug using the same method. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalJPC – Journal of Planar Chromatography – Modern TLC
Volume33
Issue number3
Early online date28 Jul 2020
DOIs
Publication statusE-pub ahead of print - 28 Jul 2020

Keywords

  • Antioxidant
  • Berberine
  • HPTLC
  • Rutin
  • Tinospora cordifolia
  • Validation

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