TY - JOUR
T1 - Development of a novel HPTLC fingerprint method for simultaneous estimation of berberine and rutin in medicinal plants and their pharmaceutical preparations followed by its application in antioxidant assay
AU - Satija, Saurabh
AU - Tambuwala, Murtaza M.
AU - Pabreja, Kavita
AU - Bakshi, Hamid A.
AU - Chellappan, Dinesh Kumar
AU - Aljabali, Alaa A.
AU - Nammi, Srinivas
AU - Singh, Thakur Gurjeet
AU - Dureja, Harish
AU - Gupta, Gaurav
AU - Dua, Kamal
AU - Mehta, Meenu
AU - Garg, Munish
PY - 2020/7/28
Y1 - 2020/7/28
N2 - The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile phase. Detection was completed densitometrically at 254 nm. The R
F estimation of berberine and rutin was observed to be 0.67 ± 0.02 and 0.47 ± 0.02, respectively. The developed HPTLC method was validated according to ICH guidelines; the method was specific, linear and accurate and can be used to determine berberine and rutin in marketed herbal preparations. The Tinospora cordifolia plant extract was further evaluated for antioxidant activity using HPTLC, and berberine was found to be more active than rutin during DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. The method was found simple, rapid, accurate, specific and robust for the analysis of berberine and rutin in crude drug using the same method. [Figure not available: see fulltext.].
AB - The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile phase. Detection was completed densitometrically at 254 nm. The R
F estimation of berberine and rutin was observed to be 0.67 ± 0.02 and 0.47 ± 0.02, respectively. The developed HPTLC method was validated according to ICH guidelines; the method was specific, linear and accurate and can be used to determine berberine and rutin in marketed herbal preparations. The Tinospora cordifolia plant extract was further evaluated for antioxidant activity using HPTLC, and berberine was found to be more active than rutin during DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. The method was found simple, rapid, accurate, specific and robust for the analysis of berberine and rutin in crude drug using the same method. [Figure not available: see fulltext.].
KW - Antioxidant
KW - Berberine
KW - HPTLC
KW - Rutin
KW - Tinospora cordifolia
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=85088707205&partnerID=8YFLogxK
UR - http://link.springer.com/10.1007/s00764-020-00035-y
U2 - 10.1007/s00764-020-00035-y
DO - 10.1007/s00764-020-00035-y
M3 - Article
SN - 0933-4173
VL - 33
SP - 313
EP - 319
JO - JPC – Journal of Planar Chromatography – Modern TLC
JF - JPC – Journal of Planar Chromatography – Modern TLC
IS - 3
ER -
