Development of a M13 bacteriophage-based SPR detection using Salmonella as a case study

Nitsara Karronuthaisiri, Ratthaphol Charlermroj, Mary Josephine Morton, Michalina Oplatowska-Stachowiak, Irene R Grant, Christopher T Elliott

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR)-based biosensor is a popular platform for real-time monitoring and sensitive detection for a myriad of targets. However, only a few studies have reported the use of bacteriophages as specific binders for SPR-based detection. This study aimed to demonstrate how filamentous M13 bacteriophages expressing 12-mer peptides can be employed in an SPR-based assay, using a Salmonella-specific bacteriophage as a model binder to detect the foodborne bacterium Salmonella. Several important factors (immobilization buffers and methods, and interaction buffers) for a successful bacteriophage-based SPR assay were optimized. As a result, a Salmonella-specific bacteriophage-based SPR assay was achieved, with very low cross reactivity with other non-target foodborne pathogens and detection limits of 8.0 × 107 and 1.3 × 107 CFU/mL for one-time and five-time immobilized sensors, respectively. This proof-of-concept study demonstrates the feasibility of using M13 bacteriophages expressing target-specific peptides as a binder in a rapid and label-free SPR assay for pathogen detection.
Original languageEnglish
Pages (from-to)214-220
Number of pages7
JournalSensors and Actuators B: Chemical
Volume190
Early online date31 Aug 2013
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Bacteriophage
  • Peptide binders
  • SPR
  • Salmonella

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