Development of a disposable potentiometric sensor for the near patient testing of plasma thiol concentrations

S Gracheva, C Livingstone, J Davis

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    The reaction of naphthoquinone with physiological thiols has been investigated as the basis of a NPT strategy for the measurement of the latter. Screen-printed carbon electrode assemblies provide an inexpensive and inherently disposable platform for the detection methodology. The key analytical parameters underpinning the selective and sensitive (0.4 uM - 1 mM) determination of reduced thiol have been assessed, and the clinical efficacy of the approach has been demonstrated through its application to the analysis of human plasma. The results have been corroborated using standard techniques, and the routes through which the system can be adopted within mainstream biomedical environments are highlighted.
    LanguageEnglish
    Pages3833-3836
    JournalAnalytical Chemistry
    Volume76
    Issue number13
    DOIs
    Publication statusPublished - Jul 2004

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    Potentiometric sensors
    Sulfhydryl Compounds
    Plasma (human)
    Plasmas
    Naphthoquinones
    Testing
    Carbon
    Electrodes

    Cite this

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    Development of a disposable potentiometric sensor for the near patient testing of plasma thiol concentrations. / Gracheva, S; Livingstone, C; Davis, J.

    In: Analytical Chemistry, Vol. 76, No. 13, 07.2004, p. 3833-3836.

    Research output: Contribution to journalArticle

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    AU - Livingstone, C

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    AB - The reaction of naphthoquinone with physiological thiols has been investigated as the basis of a NPT strategy for the measurement of the latter. Screen-printed carbon electrode assemblies provide an inexpensive and inherently disposable platform for the detection methodology. The key analytical parameters underpinning the selective and sensitive (0.4 uM - 1 mM) determination of reduced thiol have been assessed, and the clinical efficacy of the approach has been demonstrated through its application to the analysis of human plasma. The results have been corroborated using standard techniques, and the routes through which the system can be adopted within mainstream biomedical environments are highlighted.

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