Continual development in the field of Knowledge Management (KM) has led to a number of critical factors being highlighted in the literature. These issues include the macro-environment, organizational climate, technological, informational, cultural and people factors. Understanding of these issues is currently limited by a paucity of systematic empirical research addressing the relationship between the cultural and technological aspects of Knowledge Management. This paper describes the development of a conceptual model for Knowledge Management implementation, which is based on a university/industry partnership and a successful PhD study. Moreover, the paper covers theory-testing and refinement of the model using large survey empirical analysis. It is concluded that the model can be used across a wide spectrum of organizations to show how cultural and technological aspects of Knowledge Management can be systematically integrated into organizations.