Determination of total reduced thiol levels in plasma using a bromide substituted quinone

GAM Lobo, SA Chitre, SM Rathod, RB Smith, R Leslie, C Livingstone, J Davis

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Abstract

The exploitation of 2-bromo-1,4-naphthoquinone (NQBr) as a selective redox label for the determination of reduced thiol functionalities (RSH) has been investigated. The system is selective for RSH functionality, giving distinct voltammetric signals for glutathione and cysteine but can also be adapted for broad spectrum thiol detection. Ion chromatographic protocols based on conductimetric detection enable total RSH analysis (protein and monomolecular moieties) within human plasma. Bromide released through the reaction can be easily quantified and integrated within normal IC measurements. The efficacy of the approach has been assessed and the response validated through comparison with the standard colorimetric technique.
Original languageEnglish
Pages (from-to)2523-2528
JournalElectroanalysis
Volume19
Issue number24
DOIs
Publication statusPublished - Dec 2007

Keywords

  • plasma
  • thiol
  • bromide
  • ion chromatography
  • oxidative stress

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    Lobo, GAM., Chitre, SA., Rathod, SM., Smith, RB., Leslie, R., Livingstone, C., & Davis, J. (2007). Determination of total reduced thiol levels in plasma using a bromide substituted quinone. Electroanalysis, 19(24), 2523-2528. https://doi.org/10.1002/elan.200703977