Determination of total reduced thiol levels in plasma using a bromide substituted quinone

GAM Lobo, SA Chitre, SM Rathod, RB Smith, R Leslie, C Livingstone, J Davis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The exploitation of 2-bromo-1,4-naphthoquinone (NQBr) as a selective redox label for the determination of reduced thiol functionalities (RSH) has been investigated. The system is selective for RSH functionality, giving distinct voltammetric signals for glutathione and cysteine but can also be adapted for broad spectrum thiol detection. Ion chromatographic protocols based on conductimetric detection enable total RSH analysis (protein and monomolecular moieties) within human plasma. Bromide released through the reaction can be easily quantified and integrated within normal IC measurements. The efficacy of the approach has been assessed and the response validated through comparison with the standard colorimetric technique.
LanguageEnglish
Pages2523-2528
JournalElectroanalysis
Volume19
Issue number24
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Bromides
Sulfhydryl Compounds
Plasma (human)
Plasmas
Glutathione
Cysteine
Labels
Ions
Proteins
benzoquinone
Oxidation-Reduction
2-bromo-1,4-naphthoquinone

Keywords

  • plasma
  • thiol
  • bromide
  • ion chromatography
  • oxidative stress

Cite this

Lobo, GAM., Chitre, SA., Rathod, SM., Smith, RB., Leslie, R., Livingstone, C., & Davis, J. (2007). Determination of total reduced thiol levels in plasma using a bromide substituted quinone. Electroanalysis, 19(24), 2523-2528. https://doi.org/10.1002/elan.200703977
Lobo, GAM ; Chitre, SA ; Rathod, SM ; Smith, RB ; Leslie, R ; Livingstone, C ; Davis, J. / Determination of total reduced thiol levels in plasma using a bromide substituted quinone. In: Electroanalysis. 2007 ; Vol. 19, No. 24. pp. 2523-2528.
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Lobo, GAM, Chitre, SA, Rathod, SM, Smith, RB, Leslie, R, Livingstone, C & Davis, J 2007, 'Determination of total reduced thiol levels in plasma using a bromide substituted quinone', Electroanalysis, vol. 19, no. 24, pp. 2523-2528. https://doi.org/10.1002/elan.200703977

Determination of total reduced thiol levels in plasma using a bromide substituted quinone. / Lobo, GAM; Chitre, SA; Rathod, SM; Smith, RB; Leslie, R; Livingstone, C; Davis, J.

In: Electroanalysis, Vol. 19, No. 24, 12.2007, p. 2523-2528.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Determination of total reduced thiol levels in plasma using a bromide substituted quinone

AU - Lobo, GAM

AU - Chitre, SA

AU - Rathod, SM

AU - Smith, RB

AU - Leslie, R

AU - Livingstone, C

AU - Davis, J

PY - 2007/12

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AB - The exploitation of 2-bromo-1,4-naphthoquinone (NQBr) as a selective redox label for the determination of reduced thiol functionalities (RSH) has been investigated. The system is selective for RSH functionality, giving distinct voltammetric signals for glutathione and cysteine but can also be adapted for broad spectrum thiol detection. Ion chromatographic protocols based on conductimetric detection enable total RSH analysis (protein and monomolecular moieties) within human plasma. Bromide released through the reaction can be easily quantified and integrated within normal IC measurements. The efficacy of the approach has been assessed and the response validated through comparison with the standard colorimetric technique.

KW - plasma

KW - thiol

KW - bromide

KW - ion chromatography

KW - oxidative stress

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Lobo GAM, Chitre SA, Rathod SM, Smith RB, Leslie R, Livingstone C et al. Determination of total reduced thiol levels in plasma using a bromide substituted quinone. Electroanalysis. 2007 Dec;19(24):2523-2528. https://doi.org/10.1002/elan.200703977