Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry

Stephen McClean; E O'Kane; J Hillis; Franklin Smyth

Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry

Stephen McClean, E O'Kane, J Hillis, Franklin Smyth

Faculty Of Life & Health Sciences

Research output: Contribution to journal › Article

70 Citations (Scopus)

Abstract

A study is presented for the separation and determination of fifteen 1,4-benzodiazepine drugs and metabolites by capillary electrophoresis (CE) compared with high-performance liquid chromatography (HPLC). A comparison is made between the CE determination of the compounds by conventional UV detection and LC determination with electrospray ionisation (ESI) ion-trap mass spectrometry. CE is shown to provide superior separation to HPLC but the MS-MS capability of the ion-trap allows for the specific detection and determination of four of the compounds, diazepam, N'-desmethyldiazepam, oxazepam and temazepam in the hair of a patient under clinical treatment with diazepam and temazepam. Selected mixtures of drugs and metabolites are determined by CE and LC and the determination of diazepam and its metabolites by CE-UV-ESI-MS-MS is also presented. (C) 1999 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	273-291
Journal	JOURNAL OF CHROMATOGRAPHY A
Volume	838
Issue number	1-2
Publication status	Published - Apr 1999

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@article{895b70f1e52c42d9a02b0df474a7fe7a,

title = "Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry",

abstract = "A study is presented for the separation and determination of fifteen 1,4-benzodiazepine drugs and metabolites by capillary electrophoresis (CE) compared with high-performance liquid chromatography (HPLC). A comparison is made between the CE determination of the compounds by conventional UV detection and LC determination with electrospray ionisation (ESI) ion-trap mass spectrometry. CE is shown to provide superior separation to HPLC but the MS-MS capability of the ion-trap allows for the specific detection and determination of four of the compounds, diazepam, N'-desmethyldiazepam, oxazepam and temazepam in the hair of a patient under clinical treatment with diazepam and temazepam. Selected mixtures of drugs and metabolites are determined by CE and LC and the determination of diazepam and its metabolites by CE-UV-ESI-MS-MS is also presented. (C) 1999 Elsevier Science B.V. All rights reserved.",

author = "Stephen McClean and E O'Kane and J Hillis and Franklin Smyth",

note = "11th International Symposium on Capillary Electroseparation Techniques, VENICE, ITALY, OCT 04-07, 1998",

year = "1999",

month = apr,

language = "English",

volume = "838",

pages = "273--291",

journal = "JOURNAL OF CHROMATOGRAPHY A",

issn = "0021-9673",

publisher = "Elsevier",

number = "1-2",

}

Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry. / McClean, Stephen; O'Kane, E; Hillis, J; Smyth, Franklin.

In: JOURNAL OF CHROMATOGRAPHY A, Vol. 838, No. 1-2, 04.1999, p. 273-291.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry

AU - McClean, Stephen

AU - O'Kane, E

AU - Hillis, J

AU - Smyth, Franklin

N1 - 11th International Symposium on Capillary Electroseparation Techniques, VENICE, ITALY, OCT 04-07, 1998

PY - 1999/4

Y1 - 1999/4

N2 - A study is presented for the separation and determination of fifteen 1,4-benzodiazepine drugs and metabolites by capillary electrophoresis (CE) compared with high-performance liquid chromatography (HPLC). A comparison is made between the CE determination of the compounds by conventional UV detection and LC determination with electrospray ionisation (ESI) ion-trap mass spectrometry. CE is shown to provide superior separation to HPLC but the MS-MS capability of the ion-trap allows for the specific detection and determination of four of the compounds, diazepam, N'-desmethyldiazepam, oxazepam and temazepam in the hair of a patient under clinical treatment with diazepam and temazepam. Selected mixtures of drugs and metabolites are determined by CE and LC and the determination of diazepam and its metabolites by CE-UV-ESI-MS-MS is also presented. (C) 1999 Elsevier Science B.V. All rights reserved.

AB - A study is presented for the separation and determination of fifteen 1,4-benzodiazepine drugs and metabolites by capillary electrophoresis (CE) compared with high-performance liquid chromatography (HPLC). A comparison is made between the CE determination of the compounds by conventional UV detection and LC determination with electrospray ionisation (ESI) ion-trap mass spectrometry. CE is shown to provide superior separation to HPLC but the MS-MS capability of the ion-trap allows for the specific detection and determination of four of the compounds, diazepam, N'-desmethyldiazepam, oxazepam and temazepam in the hair of a patient under clinical treatment with diazepam and temazepam. Selected mixtures of drugs and metabolites are determined by CE and LC and the determination of diazepam and its metabolites by CE-UV-ESI-MS-MS is also presented. (C) 1999 Elsevier Science B.V. All rights reserved.

M3 - Article

VL - 838

SP - 273

EP - 291

JO - JOURNAL OF CHROMATOGRAPHY A

JF - JOURNAL OF CHROMATOGRAPHY A

SN - 0021-9673

IS - 1-2

ER -