Abstract
| Language | English |
|---|---|
| Pages | 273-291 |
| Journal | JOURNAL OF CHROMATOGRAPHY A |
| Volume | 838 |
| Issue number | 1-2 |
| Publication status | Published - Apr 1999 |
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Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry. / McClean, Stephen; O'Kane, E; Hillis, J; Smyth, Franklin.
In: JOURNAL OF CHROMATOGRAPHY A, Vol. 838, No. 1-2, 04.1999, p. 273-291.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry
AU - McClean, Stephen
AU - O'Kane, E
AU - Hillis, J
AU - Smyth, Franklin
N1 - 11th International Symposium on Capillary Electroseparation Techniques, VENICE, ITALY, OCT 04-07, 1998
PY - 1999/4
Y1 - 1999/4
N2 - A study is presented for the separation and determination of fifteen 1,4-benzodiazepine drugs and metabolites by capillary electrophoresis (CE) compared with high-performance liquid chromatography (HPLC). A comparison is made between the CE determination of the compounds by conventional UV detection and LC determination with electrospray ionisation (ESI) ion-trap mass spectrometry. CE is shown to provide superior separation to HPLC but the MS-MS capability of the ion-trap allows for the specific detection and determination of four of the compounds, diazepam, N'-desmethyldiazepam, oxazepam and temazepam in the hair of a patient under clinical treatment with diazepam and temazepam. Selected mixtures of drugs and metabolites are determined by CE and LC and the determination of diazepam and its metabolites by CE-UV-ESI-MS-MS is also presented. (C) 1999 Elsevier Science B.V. All rights reserved.
AB - A study is presented for the separation and determination of fifteen 1,4-benzodiazepine drugs and metabolites by capillary electrophoresis (CE) compared with high-performance liquid chromatography (HPLC). A comparison is made between the CE determination of the compounds by conventional UV detection and LC determination with electrospray ionisation (ESI) ion-trap mass spectrometry. CE is shown to provide superior separation to HPLC but the MS-MS capability of the ion-trap allows for the specific detection and determination of four of the compounds, diazepam, N'-desmethyldiazepam, oxazepam and temazepam in the hair of a patient under clinical treatment with diazepam and temazepam. Selected mixtures of drugs and metabolites are determined by CE and LC and the determination of diazepam and its metabolites by CE-UV-ESI-MS-MS is also presented. (C) 1999 Elsevier Science B.V. All rights reserved.
M3 - Article
VL - 838
SP - 273
EP - 291
JO - JOURNAL OF CHROMATOGRAPHY A
T2 - JOURNAL OF CHROMATOGRAPHY A
JF - JOURNAL OF CHROMATOGRAPHY A
SN - 0021-9673
IS - 1-2
ER -