Detection of glycated gastric inhibitory polypeptide within the intestines of diabetic obese (ob/ob) mice

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Abstract

Gastric inhibitory polypeptide (GIP) is produced within endocrine cells of the small intestine and released into the circulation upon nutrient ingestion. This study has quantified the levels of this insulinotropic peptide in the intestines of lean and diabetic obese ob/ob mice and estimated the proportion that is glycated. The total intestinal GIP concentration and content of the diabetic mice were significantly greater (p <0.01) than that of control animals. Affinity chromatographic separation and side-viewing GIP radioimmunoassay demonstrated that approx 20% of the GIP extracted from intestines of ob/ob mice was present in glycated form. Less than 2% of intestinal GIP was glycated in lean mice. In conclusion substantial quantities of glycated GIP exist within the intestines of diabetic ob/ob mice, suggesting that this may be a contributing factor to the physiological disarray of this syndrome.
LanguageEnglish
Pages167-171
JournalEndocrine
Volume16
Issue number3
Publication statusPublished - Dec 2001

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Gastric Inhibitory Polypeptide
Intestines
Endocrine Cells
Small Intestine
Radioimmunoassay
Eating
Food
Peptides

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title = "Detection of glycated gastric inhibitory polypeptide within the intestines of diabetic obese (ob/ob) mice",
abstract = "Gastric inhibitory polypeptide (GIP) is produced within endocrine cells of the small intestine and released into the circulation upon nutrient ingestion. This study has quantified the levels of this insulinotropic peptide in the intestines of lean and diabetic obese ob/ob mice and estimated the proportion that is glycated. The total intestinal GIP concentration and content of the diabetic mice were significantly greater (p <0.01) than that of control animals. Affinity chromatographic separation and side-viewing GIP radioimmunoassay demonstrated that approx 20{\%} of the GIP extracted from intestines of ob/ob mice was present in glycated form. Less than 2{\%} of intestinal GIP was glycated in lean mice. In conclusion substantial quantities of glycated GIP exist within the intestines of diabetic ob/ob mice, suggesting that this may be a contributing factor to the physiological disarray of this syndrome.",
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Detection of glycated gastric inhibitory polypeptide within the intestines of diabetic obese (ob/ob) mice. / Mooney, MH; Abdel-Wahab, Yasser; Morgan, LM; O'Harte, Finbarr; Flatt, Peter.

In: Endocrine, Vol. 16, No. 3, 12.2001, p. 167-171.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of glycated gastric inhibitory polypeptide within the intestines of diabetic obese (ob/ob) mice

AU - Mooney, MH

AU - Abdel-Wahab, Yasser

AU - Morgan, LM

AU - O'Harte, Finbarr

AU - Flatt, Peter

PY - 2001/12

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N2 - Gastric inhibitory polypeptide (GIP) is produced within endocrine cells of the small intestine and released into the circulation upon nutrient ingestion. This study has quantified the levels of this insulinotropic peptide in the intestines of lean and diabetic obese ob/ob mice and estimated the proportion that is glycated. The total intestinal GIP concentration and content of the diabetic mice were significantly greater (p <0.01) than that of control animals. Affinity chromatographic separation and side-viewing GIP radioimmunoassay demonstrated that approx 20% of the GIP extracted from intestines of ob/ob mice was present in glycated form. Less than 2% of intestinal GIP was glycated in lean mice. In conclusion substantial quantities of glycated GIP exist within the intestines of diabetic ob/ob mice, suggesting that this may be a contributing factor to the physiological disarray of this syndrome.

AB - Gastric inhibitory polypeptide (GIP) is produced within endocrine cells of the small intestine and released into the circulation upon nutrient ingestion. This study has quantified the levels of this insulinotropic peptide in the intestines of lean and diabetic obese ob/ob mice and estimated the proportion that is glycated. The total intestinal GIP concentration and content of the diabetic mice were significantly greater (p <0.01) than that of control animals. Affinity chromatographic separation and side-viewing GIP radioimmunoassay demonstrated that approx 20% of the GIP extracted from intestines of ob/ob mice was present in glycated form. Less than 2% of intestinal GIP was glycated in lean mice. In conclusion substantial quantities of glycated GIP exist within the intestines of diabetic ob/ob mice, suggesting that this may be a contributing factor to the physiological disarray of this syndrome.

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JO - Endocrine

T2 - Endocrine

JF - Endocrine

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