Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn

Franklin Smyth, T. J. P. Smyth, V. N. Ramachandran, F. O'Donnell, P. Brooks

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    This review discusses how crude extracts of selected plants are subjected to column chromatography and the resulting fractions tested for their antibacterial activity towards organisms (e.g., methicillin-resistant-Staphylococcus aureus). We discuss the bioactive column-chromatography fractions being further separated by preparative thin-layer chromatography and the resulting bands being investigated by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (LC-ESI-MS) and ESI-MSn. We then show how the resulting retention times, molecular masses, their fragmentation patterns, the Chemnet database and an in-house quinoline database are used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations do not correlate with any known compounds, thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multi-drug-resistant microorganisms. (C) 2012 Elsevier Ltd. All rights reserved.
    LanguageEnglish
    Pages46-54
    JournalTRAC-TRENDS IN ANALYTICAL CHEMISTRY
    Volume33
    DOIs
    Publication statusPublished - Mar 2012

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    Electrospray ionization
    Column chromatography
    Liquid chromatography
    Phytochemicals
    Molecular mass
    Mass spectrometry
    Ions
    Thin layer chromatography
    Methicillin
    High performance liquid chromatography
    Biological Products
    Complex Mixtures
    Pharmaceutical Preparations
    Microorganisms
    quinoline

    Cite this

    Smyth, F., Smyth, T. J. P., Ramachandran, V. N., O'Donnell, F., & Brooks, P. (2012). Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn. 33, 46-54. https://doi.org/10.1016/j.trac.2011.09.015
    Smyth, Franklin ; Smyth, T. J. P. ; Ramachandran, V. N. ; O'Donnell, F. ; Brooks, P. / Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn. 2012 ; Vol. 33. pp. 46-54.
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    Smyth, F, Smyth, TJP, Ramachandran, VN, O'Donnell, F & Brooks, P 2012, 'Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn', vol. 33, pp. 46-54. https://doi.org/10.1016/j.trac.2011.09.015

    Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn. / Smyth, Franklin; Smyth, T. J. P.; Ramachandran, V. N.; O'Donnell, F.; Brooks, P.

    Vol. 33, 03.2012, p. 46-54.

    Research output: Contribution to journalArticle

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    Smyth F, Smyth TJP, Ramachandran VN, O'Donnell F, Brooks P. Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn. 2012 Mar;33:46-54. https://doi.org/10.1016/j.trac.2011.09.015