Decreased myocardial expression of dystrophin and titin mRNA and protein in dilated cardiomyopathy: Possibly an adverse effect of TNF-α

Shamim Ahmad, Taranjit Singh Rai, Madhu Khullar, Ajay Bahl, Uma Nahar Saikia, M. Thungapathra, Rohit Manoj Kumar, Rajiv Mahajan, Kewal K. Talwar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and aims: While the molecular basis of dilated cardiomyopathy (DCM) remains uncertain, concrete evidence is emerging that sarcomeric and cytoskeleton gene expression of myocardium isolated from failing versus non-failing patients differ dramatically. The central aim to this work was to find out the possible role of dystrophin and titin along with the TNF-α in the pathogenesis of cardiomyopathy. Patients and methods: mRNA levels and protein expression of a cytoskeletal protein, dystrophin and a sarcomeric protein, titin in endomyocardial biopsies of DCM patients were examined using RT-PCR and immunohistochemistry, respectively. Further, we examined the effect of TNF-α on myocardial expression of titin and dystrophin in vitro in rat cardiac myoblast cell line (H9c2). Results: We observed significantly decreased mRNA and protein levels of dystrophin and titin in endomyocardial biopsy of DCM patients as compared to control group. The decreased levels of these proteins correlated with the severity of the disease. Plasma levels of both TNF-α and its soluble receptors TNFR1 and TNFR2 were found to be significantly higher in patients as compared to control group. Treatment of H9c2 cells with TNF-α resulted in a dose-and time-dependent decrease in mRNA levels of dystrophin and titin. Pretreatment of these cells with MG132, an inhibitor of nuclear factor kappa B (NF-κB) pathway, abolished TNF-α-induced reduction in mRNA levels of dystrophin and titin. Conclusion: Our results suggest that reduced expression of dystrophin and titin is associated with the pathophysiology of DCM, and TNF-α may modulate the expression of these proteins via NF-κB pathway.

LanguageEnglish
Pages520-530
Number of pages11
JournalJournal of Clinical Immunology
Volume30
Issue number4
DOIs
Publication statusPublished - 1 Jul 2010

Fingerprint

Connectin
Dystrophin
Dilated Cardiomyopathy
Tumor Necrosis Factor-alpha
Messenger RNA
Proteins
Cardiac Myoblasts
Receptors, Tumor Necrosis Factor, Type II
Receptors, Tumor Necrosis Factor, Type I
Biopsy
Control Groups
Cytoskeletal Proteins
NF-kappa B
Cytoskeleton
Cardiomyopathies
Myocardium
Immunohistochemistry
Gene Expression
Cell Line
Polymerase Chain Reaction

Keywords

  • Dilated cardiomyopathy
  • Dystrophin
  • Endomyocardial biopsy
  • Proinflammatory cytokines
  • Titin-mRNA expression

Cite this

Ahmad, Shamim ; Rai, Taranjit Singh ; Khullar, Madhu ; Bahl, Ajay ; Saikia, Uma Nahar ; Thungapathra, M. ; Kumar, Rohit Manoj ; Mahajan, Rajiv ; Talwar, Kewal K. / Decreased myocardial expression of dystrophin and titin mRNA and protein in dilated cardiomyopathy : Possibly an adverse effect of TNF-α. In: Journal of Clinical Immunology. 2010 ; Vol. 30, No. 4. pp. 520-530.
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abstract = "Background and aims: While the molecular basis of dilated cardiomyopathy (DCM) remains uncertain, concrete evidence is emerging that sarcomeric and cytoskeleton gene expression of myocardium isolated from failing versus non-failing patients differ dramatically. The central aim to this work was to find out the possible role of dystrophin and titin along with the TNF-α in the pathogenesis of cardiomyopathy. Patients and methods: mRNA levels and protein expression of a cytoskeletal protein, dystrophin and a sarcomeric protein, titin in endomyocardial biopsies of DCM patients were examined using RT-PCR and immunohistochemistry, respectively. Further, we examined the effect of TNF-α on myocardial expression of titin and dystrophin in vitro in rat cardiac myoblast cell line (H9c2). Results: We observed significantly decreased mRNA and protein levels of dystrophin and titin in endomyocardial biopsy of DCM patients as compared to control group. The decreased levels of these proteins correlated with the severity of the disease. Plasma levels of both TNF-α and its soluble receptors TNFR1 and TNFR2 were found to be significantly higher in patients as compared to control group. Treatment of H9c2 cells with TNF-α resulted in a dose-and time-dependent decrease in mRNA levels of dystrophin and titin. Pretreatment of these cells with MG132, an inhibitor of nuclear factor kappa B (NF-κB) pathway, abolished TNF-α-induced reduction in mRNA levels of dystrophin and titin. Conclusion: Our results suggest that reduced expression of dystrophin and titin is associated with the pathophysiology of DCM, and TNF-α may modulate the expression of these proteins via NF-κB pathway.",
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Decreased myocardial expression of dystrophin and titin mRNA and protein in dilated cardiomyopathy : Possibly an adverse effect of TNF-α. / Ahmad, Shamim; Rai, Taranjit Singh; Khullar, Madhu; Bahl, Ajay; Saikia, Uma Nahar; Thungapathra, M.; Kumar, Rohit Manoj; Mahajan, Rajiv; Talwar, Kewal K.

In: Journal of Clinical Immunology, Vol. 30, No. 4, 01.07.2010, p. 520-530.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Decreased myocardial expression of dystrophin and titin mRNA and protein in dilated cardiomyopathy

T2 - Journal of Clinical Immunology

AU - Ahmad, Shamim

AU - Rai, Taranjit Singh

AU - Khullar, Madhu

AU - Bahl, Ajay

AU - Saikia, Uma Nahar

AU - Thungapathra, M.

AU - Kumar, Rohit Manoj

AU - Mahajan, Rajiv

AU - Talwar, Kewal K.

PY - 2010/7/1

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N2 - Background and aims: While the molecular basis of dilated cardiomyopathy (DCM) remains uncertain, concrete evidence is emerging that sarcomeric and cytoskeleton gene expression of myocardium isolated from failing versus non-failing patients differ dramatically. The central aim to this work was to find out the possible role of dystrophin and titin along with the TNF-α in the pathogenesis of cardiomyopathy. Patients and methods: mRNA levels and protein expression of a cytoskeletal protein, dystrophin and a sarcomeric protein, titin in endomyocardial biopsies of DCM patients were examined using RT-PCR and immunohistochemistry, respectively. Further, we examined the effect of TNF-α on myocardial expression of titin and dystrophin in vitro in rat cardiac myoblast cell line (H9c2). Results: We observed significantly decreased mRNA and protein levels of dystrophin and titin in endomyocardial biopsy of DCM patients as compared to control group. The decreased levels of these proteins correlated with the severity of the disease. Plasma levels of both TNF-α and its soluble receptors TNFR1 and TNFR2 were found to be significantly higher in patients as compared to control group. Treatment of H9c2 cells with TNF-α resulted in a dose-and time-dependent decrease in mRNA levels of dystrophin and titin. Pretreatment of these cells with MG132, an inhibitor of nuclear factor kappa B (NF-κB) pathway, abolished TNF-α-induced reduction in mRNA levels of dystrophin and titin. Conclusion: Our results suggest that reduced expression of dystrophin and titin is associated with the pathophysiology of DCM, and TNF-α may modulate the expression of these proteins via NF-κB pathway.

AB - Background and aims: While the molecular basis of dilated cardiomyopathy (DCM) remains uncertain, concrete evidence is emerging that sarcomeric and cytoskeleton gene expression of myocardium isolated from failing versus non-failing patients differ dramatically. The central aim to this work was to find out the possible role of dystrophin and titin along with the TNF-α in the pathogenesis of cardiomyopathy. Patients and methods: mRNA levels and protein expression of a cytoskeletal protein, dystrophin and a sarcomeric protein, titin in endomyocardial biopsies of DCM patients were examined using RT-PCR and immunohistochemistry, respectively. Further, we examined the effect of TNF-α on myocardial expression of titin and dystrophin in vitro in rat cardiac myoblast cell line (H9c2). Results: We observed significantly decreased mRNA and protein levels of dystrophin and titin in endomyocardial biopsy of DCM patients as compared to control group. The decreased levels of these proteins correlated with the severity of the disease. Plasma levels of both TNF-α and its soluble receptors TNFR1 and TNFR2 were found to be significantly higher in patients as compared to control group. Treatment of H9c2 cells with TNF-α resulted in a dose-and time-dependent decrease in mRNA levels of dystrophin and titin. Pretreatment of these cells with MG132, an inhibitor of nuclear factor kappa B (NF-κB) pathway, abolished TNF-α-induced reduction in mRNA levels of dystrophin and titin. Conclusion: Our results suggest that reduced expression of dystrophin and titin is associated with the pathophysiology of DCM, and TNF-α may modulate the expression of these proteins via NF-κB pathway.

KW - Dilated cardiomyopathy

KW - Dystrophin

KW - Endomyocardial biopsy

KW - Proinflammatory cytokines

KW - Titin-mRNA expression

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