Decolourisation of synthetic and spentwash melanoidins using the white-rot fungus Phanerochaete chrysosporium JAG-40

J Dahiya, D Singh, Poonam Singh - Nee Nigam

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Phanerochaete chrysosporium JAG-40 was isolated from the soil samples saturated with spilled molasses collected from a sugar mill. This isolate decolourised synthetic and natural melanoidins present in spentwash in liquid fermentation; up to 80% in 6 days at 30 degreesC under aerobic conditions. A large inoculum size stimulated fungal biomass production, but this gave less decolourisation of pigment; 5% w/v (dry weight) mycelial suspension was found optimum for maximum decolourisation in melanoidin medium supplemented with glucose and peptone. Gel-filtration chromatography showed that larger molecular weight fractions of melanoidin were decolourised more rapidly than small molecular weight fractions. (C) 2001 Elsevier Science Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)95-98
JournalBioresource Technology
Volume78
Issue number1
Publication statusPublished - May 2001

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