D-glucose metabolism in BRIN-BD11 islet cells

J Rasschaert, Peter Flatt, CR Barnett, Neville McClenaghan, WJ Malaisse

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A novel insulin-secreting cell line, BRIN-BD11, was recently established following electrofusion of RINm5F cells with NEDH rat pancreatic islet cells. In the present study, D-glucose metabolism was compared in BRIN-BD11 and RINm5F cells. The concentration dependency of D-[5-H-3]glucose utilization displayed a comparable pattern in the two cell lines, but the absolute values were lower in BRIN-BD11 than RINm5F cells. Except in the case of D-[1-C-14]glucose, the ratio between C-14 labeled D-glucose oxidation and D-[5-H-3]glucose utilization was higher, however, in BRIN-BD11 than RINm5F cells. Moreover, BRIN-BD11 cells were less affected than RINm5F cells by a rise in D-glucose concentration, in terms of the inhibitory action of the hexose upon oxidative variables, such as oxidative glycolysis, pyruvate decarboxylation, and oxidation of glucose-derived acetyl residues in the Krebs cycle. The total energy yield from D-glucose catabolism appeared similar, however, in BRIN-BD11 and RINm5F cells. These findings extend the knowledge that BRIN-BD11 cells display an improved metabolic and secretory behavior, when considering the difference otherwise found between normal and tumoral islet cells. (C) 1996 Academic Press, Inc.
LanguageEnglish
Pages97-105
JournalBiochemical and Molecular Medicine
Volume57
Issue number2
Publication statusPublished - Apr 1996

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Islets of Langerhans
Glucose
Cell Line
Dilatation and Curettage
Decarboxylation
Hexoses
Citric Acid Cycle
Insulin-Secreting Cells
Glycolysis
Pyruvic Acid

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Rasschaert, J., Flatt, P., Barnett, CR., McClenaghan, N., & Malaisse, WJ. (1996). D-glucose metabolism in BRIN-BD11 islet cells. Biochemical and Molecular Medicine, 57(2), 97-105.
Rasschaert, J ; Flatt, Peter ; Barnett, CR ; McClenaghan, Neville ; Malaisse, WJ. / D-glucose metabolism in BRIN-BD11 islet cells. In: Biochemical and Molecular Medicine. 1996 ; Vol. 57, No. 2. pp. 97-105.
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Rasschaert, J, Flatt, P, Barnett, CR, McClenaghan, N & Malaisse, WJ 1996, 'D-glucose metabolism in BRIN-BD11 islet cells', Biochemical and Molecular Medicine, vol. 57, no. 2, pp. 97-105.

D-glucose metabolism in BRIN-BD11 islet cells. / Rasschaert, J; Flatt, Peter; Barnett, CR; McClenaghan, Neville; Malaisse, WJ.

In: Biochemical and Molecular Medicine, Vol. 57, No. 2, 04.1996, p. 97-105.

Research output: Contribution to journalArticle

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AB - A novel insulin-secreting cell line, BRIN-BD11, was recently established following electrofusion of RINm5F cells with NEDH rat pancreatic islet cells. In the present study, D-glucose metabolism was compared in BRIN-BD11 and RINm5F cells. The concentration dependency of D-[5-H-3]glucose utilization displayed a comparable pattern in the two cell lines, but the absolute values were lower in BRIN-BD11 than RINm5F cells. Except in the case of D-[1-C-14]glucose, the ratio between C-14 labeled D-glucose oxidation and D-[5-H-3]glucose utilization was higher, however, in BRIN-BD11 than RINm5F cells. Moreover, BRIN-BD11 cells were less affected than RINm5F cells by a rise in D-glucose concentration, in terms of the inhibitory action of the hexose upon oxidative variables, such as oxidative glycolysis, pyruvate decarboxylation, and oxidation of glucose-derived acetyl residues in the Krebs cycle. The total energy yield from D-glucose catabolism appeared similar, however, in BRIN-BD11 and RINm5F cells. These findings extend the knowledge that BRIN-BD11 cells display an improved metabolic and secretory behavior, when considering the difference otherwise found between normal and tumoral islet cells. (C) 1996 Academic Press, Inc.

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Rasschaert J, Flatt P, Barnett CR, McClenaghan N, Malaisse WJ. D-glucose metabolism in BRIN-BD11 islet cells. Biochemical and Molecular Medicine. 1996 Apr;57(2):97-105.