CYTOCHROME-P450-DEPENDENT MIXED-FUNCTION OXIDASE AND GLUTATHIONE-S-TRANSFERASE ACTIVITIES IN SPONTANEOUS OBESITY-DIABETES

CR Barnett, RA Abbott, CJ Bailey, Peter Flatt, C Ioannides

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The effect of non-insulin-dependent diabetes on the hepatic microsomal cytochrome P450-dependent mixed-function oxidase system and on cytosolic glutathione S-transferase activity was determined using the spontaneously obese-diabetic (ob/ob) mouse model. The activities of the xenobiotic-metabolizing cytochrome P450 proteins were monitored by the use of chemical probes. Non-insulin-dependent diabetes did not influence the hepatic metabolism of substrates associated with the P450 I, IIB. IIE. III and IV families of cytochromes. In contrast, cytosolic glutathione S-transferase activity was markedly reduced and glutathione levels were significantly lowered. These findings raise the possibility that patients suffering from this disease may be more susceptible to chemicals that rely on glutathione conjugation for their deactivation.
LanguageEnglish
Pages1868-1871
JournalBIiochemical Pharmacology
Volume43
Issue number8
Publication statusPublished - Apr 1992

Fingerprint

Mixed Function Oxygenases
Glutathione Transferase
Cytochrome P-450 Enzyme System
Glutathione
Obesity
Liver
Xenobiotics
Cytochromes
Proteins

Cite this

@article{bff4cb59181d41b680289bb8f9e087d8,
title = "CYTOCHROME-P450-DEPENDENT MIXED-FUNCTION OXIDASE AND GLUTATHIONE-S-TRANSFERASE ACTIVITIES IN SPONTANEOUS OBESITY-DIABETES",
abstract = "The effect of non-insulin-dependent diabetes on the hepatic microsomal cytochrome P450-dependent mixed-function oxidase system and on cytosolic glutathione S-transferase activity was determined using the spontaneously obese-diabetic (ob/ob) mouse model. The activities of the xenobiotic-metabolizing cytochrome P450 proteins were monitored by the use of chemical probes. Non-insulin-dependent diabetes did not influence the hepatic metabolism of substrates associated with the P450 I, IIB. IIE. III and IV families of cytochromes. In contrast, cytosolic glutathione S-transferase activity was markedly reduced and glutathione levels were significantly lowered. These findings raise the possibility that patients suffering from this disease may be more susceptible to chemicals that rely on glutathione conjugation for their deactivation.",
author = "CR Barnett and RA Abbott and CJ Bailey and Peter Flatt and C Ioannides",
year = "1992",
month = "4",
language = "English",
volume = "43",
pages = "1868--1871",
journal = "BIiochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier",
number = "8",

}

CYTOCHROME-P450-DEPENDENT MIXED-FUNCTION OXIDASE AND GLUTATHIONE-S-TRANSFERASE ACTIVITIES IN SPONTANEOUS OBESITY-DIABETES. / Barnett, CR; Abbott, RA; Bailey, CJ; Flatt, Peter; Ioannides, C.

In: BIiochemical Pharmacology, Vol. 43, No. 8, 04.1992, p. 1868-1871.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CYTOCHROME-P450-DEPENDENT MIXED-FUNCTION OXIDASE AND GLUTATHIONE-S-TRANSFERASE ACTIVITIES IN SPONTANEOUS OBESITY-DIABETES

AU - Barnett, CR

AU - Abbott, RA

AU - Bailey, CJ

AU - Flatt, Peter

AU - Ioannides, C

PY - 1992/4

Y1 - 1992/4

N2 - The effect of non-insulin-dependent diabetes on the hepatic microsomal cytochrome P450-dependent mixed-function oxidase system and on cytosolic glutathione S-transferase activity was determined using the spontaneously obese-diabetic (ob/ob) mouse model. The activities of the xenobiotic-metabolizing cytochrome P450 proteins were monitored by the use of chemical probes. Non-insulin-dependent diabetes did not influence the hepatic metabolism of substrates associated with the P450 I, IIB. IIE. III and IV families of cytochromes. In contrast, cytosolic glutathione S-transferase activity was markedly reduced and glutathione levels were significantly lowered. These findings raise the possibility that patients suffering from this disease may be more susceptible to chemicals that rely on glutathione conjugation for their deactivation.

AB - The effect of non-insulin-dependent diabetes on the hepatic microsomal cytochrome P450-dependent mixed-function oxidase system and on cytosolic glutathione S-transferase activity was determined using the spontaneously obese-diabetic (ob/ob) mouse model. The activities of the xenobiotic-metabolizing cytochrome P450 proteins were monitored by the use of chemical probes. Non-insulin-dependent diabetes did not influence the hepatic metabolism of substrates associated with the P450 I, IIB. IIE. III and IV families of cytochromes. In contrast, cytosolic glutathione S-transferase activity was markedly reduced and glutathione levels were significantly lowered. These findings raise the possibility that patients suffering from this disease may be more susceptible to chemicals that rely on glutathione conjugation for their deactivation.

M3 - Article

VL - 43

SP - 1868

EP - 1871

JO - BIiochemical Pharmacology

T2 - BIiochemical Pharmacology

JF - BIiochemical Pharmacology

SN - 0006-2952

IS - 8

ER -