Cystatin C as a biomarker for cardiorenal syndrome diseases quantitative diagnostics and monitoring via point-of-care

X. Zhang, S. Fishlock, P. Sharpe, J. Mclaughlin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

With heart failure (HF) and renal malfunction becoming global public health issues, there is an urgent need to monitor diseases at home or in the community. Point-of-care testing (POC) would shorten the patients waiting time compared with laboratory molecular analysis. This work evaluates two types of gold nanomaterials, and two assay protocols, to develop a lateral flow (LF) system for Cystatin C (CysC) quantification. Of the protocols, the 'delayed-release' shows the alleviation of the hook effects with 1% BSA running buffer (RB), albeit at increased complexity with three steps of washing. The standard method with sample dilution (1: 150 sample dilution for GNPs, and 1:10 sample dilution for GNRs) can ensure the clinical range detection of CysC as 1 mg/L with partial LF assays. GNPs have stronger optical signal intensity compared with GNRs and developed full LF assays with GNPs require 1:1.5 sample dilution in recombinant Cys C detection. The ideal sample dilution ratio is different for partial and full LF assays. Clinical Relevance - This work is the basis of future work that will use LF devices for human serum/plasma monitoring to assess kidney function related to heart failure during medication. The specificity, sensitivity, and limit of detection will be validated via a clinical trial before potential clinical use.

Original languageEnglish
Title of host publication44th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2022
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1428-1431
Number of pages4
ISBN (Electronic)9781728127828
ISBN (Print)2375-7477
DOIs
Publication statusPublished (in print/issue) - 2022
Event44th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2022 - Glasgow, United Kingdom
Duration: 11 Jul 202215 Jul 2022

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume2022-July
ISSN (Print)1557-170X

Conference

Conference44th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2022
Country/TerritoryUnited Kingdom
CityGlasgow
Period11/07/2215/07/22

Bibliographical note

Funding Information:
This project has been funded by the European Union's INTERREG VA program, managed by the Special EU Programs Body (SEUPB).

Publisher Copyright:
© 2022 IEEE.

Fingerprint

Dive into the research topics of 'Cystatin C as a biomarker for cardiorenal syndrome diseases quantitative diagnostics and monitoring via point-of-care'. Together they form a unique fingerprint.

Cite this