Cryo DualBeam Focused Ion Beam-Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds.

Edwin Lamers, X Frank Walboomers, Maciej Domanski, George McKerr, Barry O'Hagan, Clifford Barnes, Lloyd Peto, Regina Luttge, Louis A J A Winnubst, Han J G E Gardeniers, John A Jansen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

With the advance of nanotechnology in biomaterials science and tissue engineering, it is essential that new techniques become available to observe processes that take place at the direct interface between tissue and scaffold materials. Here, Cryo DualBeam focused ion beam-scanning electron microscopy (FIB-SEM) was used as a novel approach to observe the interactions between frozen hydrated cells and nanometric structures in high detail. Through a comparison of images acquired with transmission electron microscopy (TEM), conventional FIB-SEM operated at ambient temperature, and Cryo DualBeam FIB-SEM, the advantages and disadvantages of each technique were evaluated. Ultrastructural details of both (extra)cellular components and cell organelles were best observe with TEM. However, processing artifacts such as shrinkage of cells at the substrate interface were introduced in both TEM and conventional FIB-SEM. In addition, the cellular contrast in conventional FIB-SEM was low; consequently, cells were difficult to distinguish from the adjoining substrate. Cryo DualBeam FIB-SEM did preserve (extra)cellular details like the contour, cell membrane, and mineralized matrix. The three described techniques have proven to be complementary for the evaluation of processes that take place at the interface between tissue and substrate.
LanguageEnglish
Pages1-7
JournalTissue Engineering Part C Methods
Volume17
Issue number1
DOIs
Publication statusPublished - 2010

Fingerprint

Focused ion beams
Scaffolds
Electron Scanning Microscopy
Ions
Scanning electron microscopy
Transmission Electron Microscopy
Transmission electron microscopy
Substrates
Tissue
Tissue Scaffolds
Nanotechnology
Biocompatible Materials
Cellular Structures
Cell membranes
Tissue Engineering
Tissue engineering
Biomaterials
Organelles
Artifacts
Cell Membrane

Cite this

Lamers, Edwin ; Walboomers, X Frank ; Domanski, Maciej ; McKerr, George ; O'Hagan, Barry ; Barnes, Clifford ; Peto, Lloyd ; Luttge, Regina ; Winnubst, Louis A J A ; Gardeniers, Han J G E ; Jansen, John A. / Cryo DualBeam Focused Ion Beam-Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds. In: Tissue Engineering Part C Methods. 2010 ; Vol. 17, No. 1. pp. 1-7.
@article{caba6cbee48a4c87adf96bd514226456,
title = "Cryo DualBeam Focused Ion Beam-Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds.",
abstract = "With the advance of nanotechnology in biomaterials science and tissue engineering, it is essential that new techniques become available to observe processes that take place at the direct interface between tissue and scaffold materials. Here, Cryo DualBeam focused ion beam-scanning electron microscopy (FIB-SEM) was used as a novel approach to observe the interactions between frozen hydrated cells and nanometric structures in high detail. Through a comparison of images acquired with transmission electron microscopy (TEM), conventional FIB-SEM operated at ambient temperature, and Cryo DualBeam FIB-SEM, the advantages and disadvantages of each technique were evaluated. Ultrastructural details of both (extra)cellular components and cell organelles were best observe with TEM. However, processing artifacts such as shrinkage of cells at the substrate interface were introduced in both TEM and conventional FIB-SEM. In addition, the cellular contrast in conventional FIB-SEM was low; consequently, cells were difficult to distinguish from the adjoining substrate. Cryo DualBeam FIB-SEM did preserve (extra)cellular details like the contour, cell membrane, and mineralized matrix. The three described techniques have proven to be complementary for the evaluation of processes that take place at the interface between tissue and substrate.",
author = "Edwin Lamers and Walboomers, {X Frank} and Maciej Domanski and George McKerr and Barry O'Hagan and Clifford Barnes and Lloyd Peto and Regina Luttge and Winnubst, {Louis A J A} and Gardeniers, {Han J G E} and Jansen, {John A}",
year = "2010",
doi = "10.1089/ten.tec.2010.0251",
language = "English",
volume = "17",
pages = "1--7",
journal = "Tissue Engineering Part C Methods",
issn = "1937-3392",
number = "1",

}

Lamers, E, Walboomers, XF, Domanski, M, McKerr, G, O'Hagan, B, Barnes, C, Peto, L, Luttge, R, Winnubst, LAJA, Gardeniers, HJGE & Jansen, JA 2010, 'Cryo DualBeam Focused Ion Beam-Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds.', Tissue Engineering Part C Methods, vol. 17, no. 1, pp. 1-7. https://doi.org/10.1089/ten.tec.2010.0251

Cryo DualBeam Focused Ion Beam-Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds. / Lamers, Edwin; Walboomers, X Frank; Domanski, Maciej; McKerr, George; O'Hagan, Barry; Barnes, Clifford; Peto, Lloyd; Luttge, Regina; Winnubst, Louis A J A; Gardeniers, Han J G E; Jansen, John A.

In: Tissue Engineering Part C Methods, Vol. 17, No. 1, 2010, p. 1-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cryo DualBeam Focused Ion Beam-Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds.

AU - Lamers, Edwin

AU - Walboomers, X Frank

AU - Domanski, Maciej

AU - McKerr, George

AU - O'Hagan, Barry

AU - Barnes, Clifford

AU - Peto, Lloyd

AU - Luttge, Regina

AU - Winnubst, Louis A J A

AU - Gardeniers, Han J G E

AU - Jansen, John A

PY - 2010

Y1 - 2010

N2 - With the advance of nanotechnology in biomaterials science and tissue engineering, it is essential that new techniques become available to observe processes that take place at the direct interface between tissue and scaffold materials. Here, Cryo DualBeam focused ion beam-scanning electron microscopy (FIB-SEM) was used as a novel approach to observe the interactions between frozen hydrated cells and nanometric structures in high detail. Through a comparison of images acquired with transmission electron microscopy (TEM), conventional FIB-SEM operated at ambient temperature, and Cryo DualBeam FIB-SEM, the advantages and disadvantages of each technique were evaluated. Ultrastructural details of both (extra)cellular components and cell organelles were best observe with TEM. However, processing artifacts such as shrinkage of cells at the substrate interface were introduced in both TEM and conventional FIB-SEM. In addition, the cellular contrast in conventional FIB-SEM was low; consequently, cells were difficult to distinguish from the adjoining substrate. Cryo DualBeam FIB-SEM did preserve (extra)cellular details like the contour, cell membrane, and mineralized matrix. The three described techniques have proven to be complementary for the evaluation of processes that take place at the interface between tissue and substrate.

AB - With the advance of nanotechnology in biomaterials science and tissue engineering, it is essential that new techniques become available to observe processes that take place at the direct interface between tissue and scaffold materials. Here, Cryo DualBeam focused ion beam-scanning electron microscopy (FIB-SEM) was used as a novel approach to observe the interactions between frozen hydrated cells and nanometric structures in high detail. Through a comparison of images acquired with transmission electron microscopy (TEM), conventional FIB-SEM operated at ambient temperature, and Cryo DualBeam FIB-SEM, the advantages and disadvantages of each technique were evaluated. Ultrastructural details of both (extra)cellular components and cell organelles were best observe with TEM. However, processing artifacts such as shrinkage of cells at the substrate interface were introduced in both TEM and conventional FIB-SEM. In addition, the cellular contrast in conventional FIB-SEM was low; consequently, cells were difficult to distinguish from the adjoining substrate. Cryo DualBeam FIB-SEM did preserve (extra)cellular details like the contour, cell membrane, and mineralized matrix. The three described techniques have proven to be complementary for the evaluation of processes that take place at the interface between tissue and substrate.

U2 - 10.1089/ten.tec.2010.0251

DO - 10.1089/ten.tec.2010.0251

M3 - Article

VL - 17

SP - 1

EP - 7

JO - Tissue Engineering Part C Methods

T2 - Tissue Engineering Part C Methods

JF - Tissue Engineering Part C Methods

SN - 1937-3392

IS - 1

ER -