Creation of monosomic derivatives of human cultured cell lines

DJ Clarke, JF Gimenez-Abian, H Tonnies, E Neitzel, K Sperling, Stephen Downes, RT Johnson

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    Abstract

    Monosomic mammalian cell lines would be ideal for studying gene dosage effects, including gene imprinting, and for systematic isolation of recessive somatic mutants parallel to the invaluable mutants derived from haploid yeast. But autosomal monosomies are lethal in early development; although monosomies appear in tumors, deriving cell lines from these tumors is difficult and cannot provide several syngenic lines. We have developed a strategy for generating stable monosomic human cells, based on random autosomal integration of the gpt plasmid, partial inhibition of DNA topoisomerase II during mitosis to promote chromatid nondisjunction, and selection against retention of gpt, These are likely to be valuable as a source of otherwise inaccessible mutants. The strategy can also be used to generate partial mammalian monosomies, which are desirable as a source of information on recessive genes and gene imprinting.
    Original languageEnglish
    Pages (from-to)167-171
    JournalPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
    Volume95
    Issue number1
    Publication statusPublished - Jan 1998

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  • Cite this

    Clarke, DJ., Gimenez-Abian, JF., Tonnies, H., Neitzel, E., Sperling, K., Downes, S., & Johnson, RT. (1998). Creation of monosomic derivatives of human cultured cell lines. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 95(1), 167-171.