Control of lymphocytic choriomeningitis virus infection in granzyme B deficient mice

AJ Zajac, JM Dye, Daniel Quinn

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    We have investigated whether granzyme B (GzmB) is required for effective cytotoxic T lymphocyte (CTL) mediated control of lymphocytic choriomeningitis virus (LCMV) infection. Clearance of LCMV from tissues of GzmB-deficient (GzmB(-)) mice following intraperitoneal infection with LCMV was impaired compared with control mice; however, the virus was ultimately eliminated. The impaired clearance of LCMV in GzmB(-) mice was not due to a deficiency in the generation of LCMV-specific T cells. In addition, CTL from LCMV-infected GzmB(-) mice efficiently lysed virus-infected cells in vitro, but were deficient in their ability to induce rapid DNA fragmentation in target cells. We examined whether the development of protective immunity against intracranial (i.c.) rechallenge with LCMV was compromised in GzmB- mice. We found that clearance of LCMV from the brain following secondary i.c. infection also was slower in the absence of GzmB; however, the virus was ultimately eliminated and the mice survived. Our data indicate that clearance of LCMV is delayed in the absence of GzmB expression, but that other CTL effector molecules can compensate for the absence of this granule constituent in vivo. (C) 2002 Elsevier Science (USA).
    LanguageEnglish
    Pages1-9
    JournalVirology
    Volume305
    Issue number1
    DOIs
    Publication statusPublished - Jan 2003

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    Lymphocytic choriomeningitis virus
    Granzymes
    Virus Diseases
    Cytotoxic T-Lymphocytes
    Viruses
    Cercopithecine Herpesvirus 1
    DNA Fragmentation
    Coinfection
    Immunity
    T-Lymphocytes

    Cite this

    Zajac, AJ ; Dye, JM ; Quinn, Daniel. / Control of lymphocytic choriomeningitis virus infection in granzyme B deficient mice. In: Virology. 2003 ; Vol. 305, No. 1. pp. 1-9.
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    abstract = "We have investigated whether granzyme B (GzmB) is required for effective cytotoxic T lymphocyte (CTL) mediated control of lymphocytic choriomeningitis virus (LCMV) infection. Clearance of LCMV from tissues of GzmB-deficient (GzmB(-)) mice following intraperitoneal infection with LCMV was impaired compared with control mice; however, the virus was ultimately eliminated. The impaired clearance of LCMV in GzmB(-) mice was not due to a deficiency in the generation of LCMV-specific T cells. In addition, CTL from LCMV-infected GzmB(-) mice efficiently lysed virus-infected cells in vitro, but were deficient in their ability to induce rapid DNA fragmentation in target cells. We examined whether the development of protective immunity against intracranial (i.c.) rechallenge with LCMV was compromised in GzmB- mice. We found that clearance of LCMV from the brain following secondary i.c. infection also was slower in the absence of GzmB; however, the virus was ultimately eliminated and the mice survived. Our data indicate that clearance of LCMV is delayed in the absence of GzmB expression, but that other CTL effector molecules can compensate for the absence of this granule constituent in vivo. (C) 2002 Elsevier Science (USA).",
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    Control of lymphocytic choriomeningitis virus infection in granzyme B deficient mice. / Zajac, AJ; Dye, JM; Quinn, Daniel.

    In: Virology, Vol. 305, No. 1, 01.2003, p. 1-9.

    Research output: Contribution to journalArticle

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