The thermotolerant ethanol-producing yeast strain Kluyveromyces marxianus IMB3 was immobilized in calcium alginate and a 1:1 mixture of calcium alginate and the porous volcanic mineral, kissiris. Immobilized preparations were placed in fixed-bed column bioreactors and continuous ethanol production by systems containing both immobilized preparations was examined at 45 degrees C with a 100 g/l glucose feed. The effect of residence time on product concentration, bioreactor efficiency and volumetric productivities have been examined and these were all higher in systems containing the alginate/kissiris mixed immobilization matrix. Maximum ethanol concentrations produced by the continuous system ranged between 46 and 48 g/l representing efficiencies of 90-94%.
|Publication status||Published - Mar 1998|
Love, G., Gough, S., Brady, D., Barron, N., Singh - Nee Nigam, P., Singh, D., Marchant, R., & McHale, A. (1998). Continuous ethanol fermentation at 45 degrees C using Kluyveromyces marxianus IMB3 immobilized in Calcium alginate and kissiris. Bioprocess Engineering, 18(3), 187-189.