Continuous ethanol fermentation at 45 degrees C using Kluyveromyces marxianus IMB3 immobilized in Calcium alginate and kissiris

G Love, S Gough, D Brady, N Barron, P Nigam, D Singh, Roger Marchant, Anthony McHale

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The thermotolerant ethanol-producing yeast strain Kluyveromyces marxianus IMB3 was immobilized in calcium alginate and a 1:1 mixture of calcium alginate and the porous volcanic mineral, kissiris. Immobilized preparations were placed in fixed-bed column bioreactors and continuous ethanol production by systems containing both immobilized preparations was examined at 45 degrees C with a 100 g/l glucose feed. The effect of residence time on product concentration, bioreactor efficiency and volumetric productivities have been examined and these were all higher in systems containing the alginate/kissiris mixed immobilization matrix. Maximum ethanol concentrations produced by the continuous system ranged between 46 and 48 g/l representing efficiencies of 90-94%.
Original languageEnglish
Pages (from-to)187-189
JournalBioprocess Engineering
Issue number3
Publication statusPublished - Mar 1998


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