Until this research, correlation of Rosenberg’s (1965) self-esteem scale (RSES), with religious and spiritual values, was never investigated using the measure as a twofold construct instead of the monolithic form. This research paper explores the prediction of RSES by spiritual values using a twofold structure of: self-esteem-positive (SEP) and self-esteem-negative (SEN), to specify individual and fragmented correlations with spirituality, which until now was unobtainable. Confirmatory factor analysis and structural equation modelling were applied to analyse the data. The survey was conducted among two-hundred and sixty-eight participants from the Republic of Ireland. The research finds that spirituality influences peoples’ self-esteem, but clear and linear correlation between spirituality and self-esteem is difficult to be concluded. People respond oppositely and differently to positive and to negative items in the RSES which indicates that the measure is a combination of two statistically consistent constructs: SEP and SEN. The results of the study confirm that there are many spiritual areas that affect self-esteem.