Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

Ted S. Acott; Michael P. Fautsch; Weiming Mao; C. Ross Ethier; Alex S. Huang; Mary J. Kelley; Mini Aga; Sanjoy K. Bhattacharya; Terete Borras; Diane Bovenkamp; Uttio Roy Chowdhury; Abbot F. Clark; Mohammed I. Dibas; Yiqin Du; Michael H. Elliott; Jennifer A. Faralli; Haiyan Gong; Samuel Herberg; Murray A. Johnstone; Paul L. Kaufman; Kate E. Keller; Ruth A. Kelly; David Krizaj; Markus H. Kuehn; Hoi Lam Li; Raquel Lieberman; Shan C. Lin; Yutao Liu; Fiona S. McDonnell; Colleen M. McDowell; Gillian J. McLellan; Philip Mzyk; Kayarat Saidas Nair; Darryl R. Overby; Donna M. Peters; VijayKrishna Raghunathan; Ponugoti Vasantha Rao; Gavin W. Roddy; Najam A. Sharif; Myoung Sup Shim; Yang Sun; Benjamin R. Thomson; Carol B. Toris; Colin E. Willoughby; Hao F. Zhang; Thomas F. Freddo; Rudolf Fuchshofer; Kamisha R. Hill; Alireza Karimi; Krishnakumar Kizhatil; Casey C. Kopcyznski; Paloma Liton; Gaurang Patel; Michael Peng; Padmanabhan P. Pattabiraman; Ganesh Prasanna; Ester Reina-Torres; E. Griffen Samples; John R. Samples; Cynthia L. Steel; Clemens A. Strohmaier; Preeti Subramanian; Chenna Kesavulu Sugali; Joseph van Batenburg-Sherwood; Cydney Wong; Hannah Youngblood; Gulab S. Zode; Elizabeth White; W. Daniel Stamer

doi:10.1167/iovs.65.14.32

Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

Ted S. Acott
, Michael P. Fautsch
, Weiming Mao
, C. Ross Ethier
, Alex S. Huang
, Mary J. Kelley
, Mini Aga
, Sanjoy K. Bhattacharya
, Terete Borras
, Diane Bovenkamp
, Uttio Roy Chowdhury
, Abbot F. Clark
, Mohammed I. Dibas
, Yiqin Du
, Michael H. Elliott
, Jennifer A. Faralli
, Haiyan Gong
, Samuel Herberg
, Murray A. Johnstone
, Paul L. Kaufman

Kate E. Keller, Ruth A. Kelly, David Krizaj, Markus H. Kuehn, Hoi Lam Li, Raquel Lieberman, Shan C. Lin, Yutao Liu, Fiona S. McDonnell, Colleen M. McDowell, Gillian J. McLellan, Philip Mzyk, Kayarat Saidas Nair, Darryl R. Overby, Donna M. Peters, VijayKrishna Raghunathan, Ponugoti Vasantha Rao, Gavin W. Roddy, Najam A. Sharif, Myoung Sup Shim, Yang Sun, Benjamin R. Thomson, Carol B. Toris, Colin E. Willoughby, Hao F. Zhang, Thomas F. Freddo, Rudolf Fuchshofer, Kamisha R. Hill, Alireza Karimi, Krishnakumar Kizhatil, Casey C. Kopcyznski, Paloma Liton, Gaurang Patel, Michael Peng, Padmanabhan P. Pattabiraman, Ganesh Prasanna, Ester Reina-Torres, E. Griffen Samples, John R. Samples, Cynthia L. Steel, Clemens A. Strohmaier, Preeti Subramanian, Chenna Kesavulu Sugali, Joseph van Batenburg-Sherwood, Cydney Wong, Hannah Youngblood, Gulab S. Zode, Elizabeth White, W. Daniel Stamer

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Abstract

Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.

Original language	English
Article number	32
Pages (from-to)	1-32
Number of pages	32
Journal	Investigative Ophthalmology and Visual Science
Volume	65
Issue number	14
DOIs	https://doi.org/10.1167/iovs.65.14.32
Publication status	Published (in print/issue) - 31 Dec 2024

Bibliographical note

Publisher Copyright:
Copyright 2024 The Authors.

Funding

Supported by grants from the National Eye Institute, National Institutes of Health (EY030238 to TSA; EY025721 to TSA; EY010572 to TSA and KEK; EY026048 to TSA and VKR; EY008247 to TSA; EY003279 to TSA; EY021800 to MJK; EY031071 to MJK; EY030501 to ASH; EY033015 to KSN; EY026529 to CMM; EY031631 to YL; EY033961 to YL; EY023242 to YL; EY032960 to YL; EY028671 to YL; EY014800 to DK; EY027920 to DK; EY031817 to DK; EY002423 to DK; EY021205 to RLL; EY026885 to PL; EY033600 to PL; EY034096 to SH; EY022634 to HG; EY025643 to YD; EY026962 to WM; EY031700 to WM; EY033929 to WM; EY036011 to AK); The BrightFocus Foundation; Research to Prevent Blindness; Aerie Pharmaceuticals; Glaukos Corporation; Glaucoma Research Foundation; Western Glaucoma Foundation; Lewis Rudin Glaucoma Prize to MJK); and Allergan by AbbVie

Funders	Funder number
Research to Prevent Blindness
National Institutes of Health	EY026529, EY026048, EY026885, EY026962, EY031817, EY033600, EY008247, EY033961, EY003279, EY014800, EY002423, EY030238, EY031700, EY034096, EY036011, EY021800, EY022634, EY021205, EY027920, EY025721, EY025643, EY023242, EY033929, EY031631, EY028671, EY032960, EY033015, EY030501, EY010572, EY031071
National Institutes of Health

Keywords

intraocular pressure regulation models
perfused anterior segment organ culture
ex vivo aqueous humor outflow regulation models
Humans
Anterior Eye Segment
Glaucoma/physiopathology
Consensus
Animals
Intraocular Pressure/physiology
Aqueous Humor/physiology
Perfusion/methods
Trabecular Meshwork
Organ Culture Techniques

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10.1167/iovs.65.14.32Licence: CC BY-NC-ND

i1552-5783-65-14-32_1734514646.88791Final published version, 3.37 MBLicence: CC BY-NC-ND

Cite this

Acott, T. S., Fautsch, M. P., Mao, W., Ethier, C. R., Huang, A. S., Kelley, M. J., Aga, M., Bhattacharya, S. K., Borras, T., Bovenkamp, D., Chowdhury, U. R., Clark, A. F., Dibas, M. I., Du, Y., Elliott, M. H., Faralli, J. A., Gong, H., Herberg, S., Johnstone, M. A., ... Stamer, W. D. (2024). Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches. Investigative Ophthalmology and Visual Science, 65(14), 1-32. Article 32. https://doi.org/10.1167/iovs.65.14.32

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title = "Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches",

abstract = "Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.",

keywords = "intraocular pressure regulation models, perfused anterior segment organ culture, ex vivo aqueous humor outflow regulation models, Humans, Anterior Eye Segment, Glaucoma/physiopathology, Consensus, Animals, Intraocular Pressure/physiology, Aqueous Humor/physiology, Perfusion/methods, Trabecular Meshwork, Organ Culture Techniques",

author = "Acott, \{Ted S.\} and Fautsch, \{Michael P.\} and Weiming Mao and Ethier, \{C. Ross\} and Huang, \{Alex S.\} and Kelley, \{Mary J.\} and Mini Aga and Bhattacharya, \{Sanjoy K.\} and Terete Borras and Diane Bovenkamp and Chowdhury, \{Uttio Roy\} and Clark, \{Abbot F.\} and Dibas, \{Mohammed I.\} and Yiqin Du and Elliott, \{Michael H.\} and Faralli, \{Jennifer A.\} and Haiyan Gong and Samuel Herberg and Johnstone, \{Murray A.\} and Kaufman, \{Paul L.\} and Keller, \{Kate E.\} and Kelly, \{Ruth A.\} and David Krizaj and Kuehn, \{Markus H.\} and Li, \{Hoi Lam\} and Raquel Lieberman and Lin, \{Shan C.\} and Yutao Liu and McDonnell, \{Fiona S.\} and McDowell, \{Colleen M.\} and McLellan, \{Gillian J.\} and Philip Mzyk and Nair, \{Kayarat Saidas\} and Overby, \{Darryl R.\} and Peters, \{Donna M.\} and VijayKrishna Raghunathan and Rao, \{Ponugoti Vasantha\} and Roddy, \{Gavin W.\} and Sharif, \{Najam A.\} and Shim, \{Myoung Sup\} and Yang Sun and Thomson, \{Benjamin R.\} and Toris, \{Carol B.\} and Willoughby, \{Colin E.\} and Zhang, \{Hao F.\} and Freddo, \{Thomas F.\} and Rudolf Fuchshofer and Hill, \{Kamisha R.\} and Alireza Karimi and Krishnakumar Kizhatil and Kopcyznski, \{Casey C.\} and Paloma Liton and Gaurang Patel and Michael Peng and Pattabiraman, \{Padmanabhan P.\} and Ganesh Prasanna and Ester Reina-Torres and Samples, \{E. Griffen\} and Samples, \{John R.\} and Steel, \{Cynthia L.\} and Strohmaier, \{Clemens A.\} and Preeti Subramanian and Sugali, \{Chenna Kesavulu\} and \{van Batenburg-Sherwood\}, Joseph and Cydney Wong and Hannah Youngblood and Zode, \{Gulab S.\} and Elizabeth White and Stamer, \{W. Daniel\}",

year = "2024",

month = dec,

day = "31",

doi = "10.1167/iovs.65.14.32",

language = "English",

volume = "65",

pages = "1--32",

journal = "Investigative Ophthalmology and Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology Inc.",

number = "14",

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Acott, TS, Fautsch, MP, Mao, W, Ethier, CR, Huang, AS, Kelley, MJ, Aga, M, Bhattacharya, SK, Borras, T, Bovenkamp, D, Chowdhury, UR, Clark, AF, Dibas, MI, Du, Y, Elliott, MH, Faralli, JA, Gong, H, Herberg, S, Johnstone, MA, Kaufman, PL, Keller, KE, Kelly, RA, Krizaj, D, Kuehn, MH, Li, HL, Lieberman, R, Lin, SC, Liu, Y, McDonnell, FS, McDowell, CM, McLellan, GJ, Mzyk, P, Nair, KS, Overby, DR, Peters, DM, Raghunathan, V, Rao, PV, Roddy, GW, Sharif, NA, Shim, MS, Sun, Y, Thomson, BR, Toris, CB, Willoughby, CE, Zhang, HF, Freddo, TF, Fuchshofer, R, Hill, KR, Karimi, A, Kizhatil, K, Kopcyznski, CC, Liton, P, Patel, G, Peng, M, Pattabiraman, PP, Prasanna, G, Reina-Torres, E, Samples, EG, Samples, JR, Steel, CL, Strohmaier, CA, Subramanian, P, Sugali, CK, van Batenburg-Sherwood, J, Wong, C, Youngblood, H, Zode, GS, White, E & Stamer, WD 2024, 'Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches', Investigative Ophthalmology and Visual Science, vol. 65, no. 14, 32, pp. 1-32. https://doi.org/10.1167/iovs.65.14.32

TY - JOUR

T1 - Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

AU - Acott, Ted S.

AU - Fautsch, Michael P.

AU - Mao, Weiming

AU - Ethier, C. Ross

AU - Huang, Alex S.

AU - Kelley, Mary J.

AU - Aga, Mini

AU - Bhattacharya, Sanjoy K.

AU - Borras, Terete

AU - Bovenkamp, Diane

AU - Chowdhury, Uttio Roy

AU - Clark, Abbot F.

AU - Dibas, Mohammed I.

AU - Du, Yiqin

AU - Elliott, Michael H.

AU - Faralli, Jennifer A.

AU - Gong, Haiyan

AU - Herberg, Samuel

AU - Johnstone, Murray A.

AU - Kaufman, Paul L.

AU - Keller, Kate E.

AU - Kelly, Ruth A.

AU - Krizaj, David

AU - Kuehn, Markus H.

AU - Li, Hoi Lam

AU - Lieberman, Raquel

AU - Lin, Shan C.

AU - Liu, Yutao

AU - McDonnell, Fiona S.

AU - McDowell, Colleen M.

AU - McLellan, Gillian J.

AU - Mzyk, Philip

AU - Nair, Kayarat Saidas

AU - Overby, Darryl R.

AU - Peters, Donna M.

AU - Raghunathan, VijayKrishna

AU - Rao, Ponugoti Vasantha

AU - Roddy, Gavin W.

AU - Sharif, Najam A.

AU - Shim, Myoung Sup

AU - Sun, Yang

AU - Thomson, Benjamin R.

AU - Toris, Carol B.

AU - Willoughby, Colin E.

AU - Zhang, Hao F.

AU - Freddo, Thomas F.

AU - Fuchshofer, Rudolf

AU - Hill, Kamisha R.

AU - Karimi, Alireza

AU - Kizhatil, Krishnakumar

AU - Kopcyznski, Casey C.

AU - Liton, Paloma

AU - Patel, Gaurang

AU - Peng, Michael

AU - Pattabiraman, Padmanabhan P.

AU - Prasanna, Ganesh

AU - Reina-Torres, Ester

AU - Samples, E. Griffen

AU - Samples, John R.

AU - Steel, Cynthia L.

AU - Strohmaier, Clemens A.

AU - Subramanian, Preeti

AU - Sugali, Chenna Kesavulu

AU - van Batenburg-Sherwood, Joseph

AU - Wong, Cydney

AU - Youngblood, Hannah

AU - Zode, Gulab S.

AU - White, Elizabeth

AU - Stamer, W. Daniel

PY - 2024/12/31

Y1 - 2024/12/31

N2 - Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.

AB - Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.

KW - intraocular pressure regulation models

KW - perfused anterior segment organ culture

KW - ex vivo aqueous humor outflow regulation models

KW - Humans

KW - Anterior Eye Segment

KW - Glaucoma/physiopathology

KW - Consensus

KW - Animals

KW - Intraocular Pressure/physiology

KW - Aqueous Humor/physiology

KW - Perfusion/methods

KW - Trabecular Meshwork

KW - Organ Culture Techniques

UR - https://www.scopus.com/pages/publications/85212880418

U2 - 10.1167/iovs.65.14.32

DO - 10.1167/iovs.65.14.32

M3 - Article

C2 - 39693082

SN - 0146-0404

VL - 65

SP - 1

EP - 32

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

IS - 14

M1 - 32

ER -

Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

Abstract

Bibliographical note

Funding

Keywords

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