TY - JOUR
T1 - Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches
AU - Acott, Ted S.
AU - Fautsch, Michael P.
AU - Mao, Weiming
AU - Ethier, C. Ross
AU - Huang, Alex S.
AU - Kelley, Mary J.
AU - Aga, Mini
AU - Bhattacharya, Sanjoy K.
AU - Borras, Terete
AU - Bovenkamp, Diane
AU - Chowdhury, Uttio Roy
AU - Clark, Abbot F.
AU - Dibas, Mohammed I.
AU - Du, Yiqin
AU - Elliott, Michael H.
AU - Faralli, Jennifer A.
AU - Gong, Haiyan
AU - Herberg, Samuel
AU - Johnstone, Murray A.
AU - Kaufman, Paul L.
AU - Keller, Kate E.
AU - Kelly, Ruth A.
AU - Krizaj, David
AU - Kuehn, Markus H.
AU - Li, Hoi Lam
AU - Lieberman, Raquel
AU - Lin, Shan C.
AU - Liu, Yutao
AU - McDonnell, Fiona S.
AU - McDowell, Colleen M.
AU - McLellan, Gillian J.
AU - Mzyk, Philip
AU - Nair, Kayarat Saidas
AU - Overby, Darryl R.
AU - Peters, Donna M.
AU - Raghunathan, VijayKrishna
AU - Rao, Ponugoti Vasantha
AU - Roddy, Gavin W.
AU - Sharif, Najam A.
AU - Shim, Myoung Sup
AU - Sun, Yang
AU - Thomson, Benjamin R.
AU - Toris, Carol B.
AU - Willoughby, Colin E.
AU - Zhang, Hao F.
AU - Freddo, Thomas F.
AU - Fuchshofer, Rudolf
AU - Hill, Kamisha R.
AU - Karimi, Alireza
AU - Kizhatil, Krishnakumar
AU - Kopcyznski, Casey C.
AU - Liton, Paloma
AU - Patel, Gaurang
AU - Peng, Michael
AU - Pattabiraman, Padmanabhan P.
AU - Prasanna, Ganesh
AU - Reina-Torres, Ester
AU - Samples, E. Griffen
AU - Samples, John R.
AU - Steel, Cynthia L.
AU - Strohmaier, Clemens A.
AU - Subramanian, Preeti
AU - Sugali, Chenna Kesavulu
AU - van Batenburg-Sherwood, Joseph
AU - Wong, Cydney
AU - Youngblood, Hannah
AU - Zode, Gulab S.
AU - White, Elizabeth
AU - Stamer, W. Daniel
N1 - Publisher Copyright:
Copyright 2024 The Authors.
PY - 2024/12/31
Y1 - 2024/12/31
N2 - Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
AB - Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
KW - intraocular pressure regulation models
KW - perfused anterior segment organ culture
KW - ex vivo aqueous humor outflow regulation models
KW - Humans
KW - Anterior Eye Segment
KW - Glaucoma/physiopathology
KW - Consensus
KW - Animals
KW - Intraocular Pressure/physiology
KW - Aqueous Humor/physiology
KW - Perfusion/methods
KW - Trabecular Meshwork
KW - Organ Culture Techniques
UR - http://www.scopus.com/inward/record.url?scp=85212880418&partnerID=8YFLogxK
U2 - 10.1167/iovs.65.14.32
DO - 10.1167/iovs.65.14.32
M3 - Article
C2 - 39693082
SN - 0146-0404
VL - 65
SP - 1
EP - 32
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 14
M1 - 32
ER -