Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide.

KC Venneti, JP Malthouse, Finbarr O'Harte, CM Hewage

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Glucose-dependent insulinotropic polypeptide (GIP) is an insulinotropic incretin hormone that stimulates insulin secretion during a meal. GIP has glucose lowering abilities and hence is considered as a potential target molecule for type 2 diabetes therapy. In this article, we present the solution structure of GIP in membrane-mimicking environments by proton NMR spectroscopy and molecular modelling. GIP adopts an α-helical conformation between residues Phe(6)-Gly(31) and Ala(13)-Gln(29) for micellar and bicellar media, respectively. Previously we examined the effect of N-terminal Ala substitution in GIP, but here eight GIP analogues were synthesised by replacing individual residues within the central 8-18 region with alanine. These studies showed relatively minor changes in biological activity as assessed by insulin releasing potency. However, at higher concentration, GIP(Ala(16)), and GIP(Ala(18)) showed insulin secreting activity higher than the native GIP (P
Original languageEnglish
Pages (from-to)882-888
JournalBiochim Biophys Acta.
Issue number7
Publication statusPublished (in print/issue) - 1 Jul 2011


Dive into the research topics of 'Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide.'. Together they form a unique fingerprint.

Cite this