Complete genome of Escherichia coli sequence type 73 with acquired blaTEM-1 and high genotypic virulence load identified in human saliva

Francesca McDonagh; Anna Tumeo; Elaine K. Murray; Brian Hallahan; Georgios Miliotis

doi:10.1016/j.jgar.2024.08.011

Complete genome of Escherichia coli sequence type 73 with acquired blaTEM-1 and high genotypic virulence load identified in human saliva

Francesca McDonagh
, Anna Tumeo
, Elaine K. Murray
, Brian Hallahan
, Georgios Miliotis

Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway, Ireland

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

66 Downloads (Pure)

Abstract

Objectives

Escherichia coli sequence type (ST) 73 is a pandemic lineage of the ExPEC (Extraintestinal Pathogenic E. coli) family associated with conserved virulence. We report the complete genome of a genomically hypervirulent E. coli ST73 strain isolated from the oral cavity of a patient with a diagnosis of treatment resistant schizophrenia and receiving clozapine treatment.

Methods

E. coli strain GABEEC132 underwent second and third generation sequencing with Illumina and Oxford-Nanopore-Technologies (ONT) platforms. Antibiotic resistance genes (ARGs) and virulence factors (VFs) were bioinformatically identified using the NCBI-AMR-Finder-Plus database and Virulence-Factors-database (VFDB), respectively. To contextualize the genome within a broader epidemiological framework, phylogenetic analysis was conducted using representative genomes of E. coli ST73 O6:H1 (n = 55).

Results

E. coli strain GABEEC132 was identified as possessing the O6:H1 serotype and classified within the B2 phylogroup. The strain exhibited a high genomic virulence load, encoding for 194 VFs. Additionally, it encoded three ARGs, including an acquired blaTEM-1 located on a rep_cluster_2350 8 237 Kb mobilisable plasmid, presenting phenotypic resistance to ampicillin and piperacillin.

Conclusion

This report provides novel insights into the oral prevalence of genotypically hypervirulent and drug-resistant E. coli ST73, a pandemic lineage.

Original language	English
Pages (from-to)	149-152
Number of pages	4
Journal	Journal of Global Antimicrobial Resistance
Volume	39
Early online date	19 Sept 2024
DOIs	https://doi.org/10.1016/j.jgar.2024.08.011
Publication status	Published (in print/issue) - 31 Dec 2024

Bibliographical note

Data Availability Statement

Complete genome sequences of E. coli GABEEC132 has been deposited in the GenBank database under accession numbers CP157262 - CP157264 and BioProject number PRJNA1115063.

Funding

The research conducted in this publication was funded by the Irish Research Council under grant number GOIPG/2023/4515.

Funders	Funder number
University Hospital Galway
Irish Research Council	GOIPG/2023/4515
Irish Research Council

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Escherichia coli
blaTEM-1
Virulance
Saliva

Access to Document

10.1016/j.jgar.2024.08.011Licence: CC BY

1-s2.0-S2213716524001693-mainFinal published version, 868 KBLicence: CC BY

Cite this

@article{0a3827a92b6c4a09bdaf05f085d124ac,

title = "Complete genome of Escherichia coli sequence type 73 with acquired blaTEM-1 and high genotypic virulence load identified in human saliva",

abstract = "ObjectivesEscherichia coli sequence type (ST) 73 is a pandemic lineage of the ExPEC (Extraintestinal Pathogenic E. coli) family associated with conserved virulence. We report the complete genome of a genomically hypervirulent E. coli ST73 strain isolated from the oral cavity of a patient with a diagnosis of treatment resistant schizophrenia and receiving clozapine treatment.MethodsE. coli strain GABEEC132 underwent second and third generation sequencing with Illumina and Oxford-Nanopore-Technologies (ONT) platforms. Antibiotic resistance genes (ARGs) and virulence factors (VFs) were bioinformatically identified using the NCBI-AMR-Finder-Plus database and Virulence-Factors-database (VFDB), respectively. To contextualize the genome within a broader epidemiological framework, phylogenetic analysis was conducted using representative genomes of E. coli ST73 O6:H1 (n = 55).ResultsE. coli strain GABEEC132 was identified as possessing the O6:H1 serotype and classified within the B2 phylogroup. The strain exhibited a high genomic virulence load, encoding for 194 VFs. Additionally, it encoded three ARGs, including an acquired blaTEM-1 located on a rep\_cluster\_2350 8 237 Kb mobilisable plasmid, presenting phenotypic resistance to ampicillin and piperacillin.ConclusionThis report provides novel insights into the oral prevalence of genotypically hypervirulent and drug-resistant E. coli ST73, a pandemic lineage.",

keywords = "Escherichia coli, blaTEM-1, Virulance, Saliva",

author = "Francesca McDonagh and Anna Tumeo and Murray, \{Elaine K.\} and Brian Hallahan and Georgios Miliotis",

note = "Copyright {\textcopyright} 2024. Published by Elsevier Ltd.",

year = "2024",

month = dec,

day = "31",

doi = "10.1016/j.jgar.2024.08.011",

language = "English",

volume = "39",

pages = "149--152",

journal = "Journal of Global Antimicrobial Resistance",

issn = "2213-7165",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Complete genome of Escherichia coli sequence type 73 with acquired blaTEM-1 and high genotypic virulence load identified in human saliva

AU - McDonagh, Francesca

AU - Tumeo, Anna

AU - Murray, Elaine K.

AU - Hallahan, Brian

AU - Miliotis, Georgios

PY - 2024/12/31

Y1 - 2024/12/31

N2 - ObjectivesEscherichia coli sequence type (ST) 73 is a pandemic lineage of the ExPEC (Extraintestinal Pathogenic E. coli) family associated with conserved virulence. We report the complete genome of a genomically hypervirulent E. coli ST73 strain isolated from the oral cavity of a patient with a diagnosis of treatment resistant schizophrenia and receiving clozapine treatment.MethodsE. coli strain GABEEC132 underwent second and third generation sequencing with Illumina and Oxford-Nanopore-Technologies (ONT) platforms. Antibiotic resistance genes (ARGs) and virulence factors (VFs) were bioinformatically identified using the NCBI-AMR-Finder-Plus database and Virulence-Factors-database (VFDB), respectively. To contextualize the genome within a broader epidemiological framework, phylogenetic analysis was conducted using representative genomes of E. coli ST73 O6:H1 (n = 55).ResultsE. coli strain GABEEC132 was identified as possessing the O6:H1 serotype and classified within the B2 phylogroup. The strain exhibited a high genomic virulence load, encoding for 194 VFs. Additionally, it encoded three ARGs, including an acquired blaTEM-1 located on a rep_cluster_2350 8 237 Kb mobilisable plasmid, presenting phenotypic resistance to ampicillin and piperacillin.ConclusionThis report provides novel insights into the oral prevalence of genotypically hypervirulent and drug-resistant E. coli ST73, a pandemic lineage.

AB - ObjectivesEscherichia coli sequence type (ST) 73 is a pandemic lineage of the ExPEC (Extraintestinal Pathogenic E. coli) family associated with conserved virulence. We report the complete genome of a genomically hypervirulent E. coli ST73 strain isolated from the oral cavity of a patient with a diagnosis of treatment resistant schizophrenia and receiving clozapine treatment.MethodsE. coli strain GABEEC132 underwent second and third generation sequencing with Illumina and Oxford-Nanopore-Technologies (ONT) platforms. Antibiotic resistance genes (ARGs) and virulence factors (VFs) were bioinformatically identified using the NCBI-AMR-Finder-Plus database and Virulence-Factors-database (VFDB), respectively. To contextualize the genome within a broader epidemiological framework, phylogenetic analysis was conducted using representative genomes of E. coli ST73 O6:H1 (n = 55).ResultsE. coli strain GABEEC132 was identified as possessing the O6:H1 serotype and classified within the B2 phylogroup. The strain exhibited a high genomic virulence load, encoding for 194 VFs. Additionally, it encoded three ARGs, including an acquired blaTEM-1 located on a rep_cluster_2350 8 237 Kb mobilisable plasmid, presenting phenotypic resistance to ampicillin and piperacillin.ConclusionThis report provides novel insights into the oral prevalence of genotypically hypervirulent and drug-resistant E. coli ST73, a pandemic lineage.

KW - Escherichia coli

KW - blaTEM-1

KW - Virulance

KW - Saliva

UR - https://www.scopus.com/pages/publications/85206145323

U2 - 10.1016/j.jgar.2024.08.011

DO - 10.1016/j.jgar.2024.08.011

M3 - Article

C2 - 39303870

SN - 2213-7165

VL - 39

SP - 149

EP - 152

JO - Journal of Global Antimicrobial Resistance

JF - Journal of Global Antimicrobial Resistance

ER -

Complete genome of Escherichia coli sequence type 73 with acquired blaTEM-1 and high genotypic virulence load identified in human saliva

Abstract

Bibliographical note

Data Availability Statement

Funding

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this