Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells

JF Gimenez-Abian, DJ Clarke, C de la Torre, G Gimenez-Martin, AM Mullinger, Stephen Downes, RT Johnson

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase, Still, core assembly can be induced in G(1) and G(2) when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.
    LanguageEnglish
    Pages601-603
    JournalEuropean Journal of Cell Biology
    Volume78
    Issue number8
    Publication statusPublished - Aug 1999

    Fingerprint

    Chromatids
    S Phase
    Mental Competency
    Siblings
    DNA Replication
    Chromosomes
    Prometaphase
    Proteins
    Aptitude
    Interphase
    Epigenomics
    Chromatin
    Cell Cycle
    Maintenance
    Organizations

    Cite this

    Gimenez-Abian, JF., Clarke, DJ., de la Torre, C., Gimenez-Martin, G., Mullinger, AM., Downes, S., & Johnson, RT. (1999). Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells. 78(8), 601-603.
    Gimenez-Abian, JF ; Clarke, DJ ; de la Torre, C ; Gimenez-Martin, G ; Mullinger, AM ; Downes, Stephen ; Johnson, RT. / Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells. 1999 ; Vol. 78, No. 8. pp. 601-603.
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    abstract = "Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase, Still, core assembly can be induced in G(1) and G(2) when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.",
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    Gimenez-Abian, JF, Clarke, DJ, de la Torre, C, Gimenez-Martin, G, Mullinger, AM, Downes, S & Johnson, RT 1999, 'Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells', vol. 78, no. 8, pp. 601-603.

    Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells. / Gimenez-Abian, JF; Clarke, DJ; de la Torre, C; Gimenez-Martin, G; Mullinger, AM; Downes, Stephen; Johnson, RT.

    Vol. 78, No. 8, 08.1999, p. 601-603.

    Research output: Contribution to journalArticle

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    T1 - Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells

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    AU - Clarke, DJ

    AU - de la Torre, C

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    AU - Mullinger, AM

    AU - Downes, Stephen

    AU - Johnson, RT

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    N2 - Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase, Still, core assembly can be induced in G(1) and G(2) when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.

    AB - Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase, Still, core assembly can be induced in G(1) and G(2) when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.

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    Gimenez-Abian JF, Clarke DJ, de la Torre C, Gimenez-Martin G, Mullinger AM, Downes S et al. Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells. 1999 Aug;78(8):601-603.