Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health.

Emma Brown, Sonja Nitecki, Gemma Pereira-Caro, Gordon Mc Dougall, Derek Stewart, Alan Crozier, ian Rowland, Chris IR Gill

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P <0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P <0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.
    LanguageEnglish
    Pages611-623
    JournalBiofactors.
    Volume40
    Issue number6
    Early online date30 Oct 2014
    DOIs
    Publication statusPublished - Nov 2014

    Fingerprint

    Vaccinium vitis-idaea
    Polyphenols
    Digestion
    Health
    Gallic Acid
    Fermentation
    Proanthocyanidins
    Ileostomy
    Comet Assay
    Anthocyanins
    Phytochemicals
    Mutation Rate
    Colorectal Neoplasms
    Cell Culture Techniques
    High Pressure Liquid Chromatography
    In Vitro Techniques

    Keywords

    • Polyphenols
    • in vitro digestion
    • ileal fluid
    • diet
    • gut health
    • lingonberries
    • DNA damage
    • in vivo digestion

    Cite this

    Brown, Emma ; Nitecki, Sonja ; Pereira-Caro, Gemma ; Mc Dougall, Gordon ; Stewart, Derek ; Crozier, Alan ; Rowland, ian ; Gill, Chris IR. / Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health. In: Biofactors. 2014 ; Vol. 40, No. 6. pp. 611-623.
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    abstract = "The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P <0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P <0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.",
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    author = "Emma Brown and Sonja Nitecki and Gemma Pereira-Caro and {Mc Dougall}, Gordon and Derek Stewart and Alan Crozier and ian Rowland and Gill, {Chris IR}",
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    Brown, E, Nitecki, S, Pereira-Caro, G, Mc Dougall, G, Stewart, D, Crozier, A, Rowland, I & Gill, CIR 2014, 'Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health.', Biofactors., vol. 40, no. 6, pp. 611-623. https://doi.org/10.1002/biof.1173

    Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health. / Brown, Emma; Nitecki, Sonja; Pereira-Caro, Gemma; Mc Dougall, Gordon; Stewart, Derek; Crozier, Alan; Rowland, ian; Gill, Chris IR.

    In: Biofactors., Vol. 40, No. 6, 11.2014, p. 611-623.

    Research output: Contribution to journalArticle

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    T1 - Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health.

    AU - Brown, Emma

    AU - Nitecki, Sonja

    AU - Pereira-Caro, Gemma

    AU - Mc Dougall, Gordon

    AU - Stewart, Derek

    AU - Crozier, Alan

    AU - Rowland, ian

    AU - Gill, Chris IR

    PY - 2014/11

    Y1 - 2014/11

    N2 - The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P <0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P <0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.

    AB - The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P <0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P <0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.

    KW - Polyphenols

    KW - in vitro digestion

    KW - ileal fluid

    KW - diet

    KW - gut health

    KW - lingonberries

    KW - DNA damage

    KW - in vivo digestion

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