Abstract
| Language | English |
|---|---|
| Pages | 611-623 |
| Journal | Biofactors. |
| Volume | 40 |
| Issue number | 6 |
| Early online date | 30 Oct 2014 |
| DOIs | |
| Publication status | Published - Nov 2014 |
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Keywords
- Polyphenols
- in vitro digestion
- ileal fluid
- diet
- gut health
- lingonberries
- DNA damage
- in vivo digestion
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Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health. / Brown, Emma; Nitecki, Sonja; Pereira-Caro, Gemma; Mc Dougall, Gordon; Stewart, Derek; Crozier, Alan; Rowland, ian; Gill, Chris IR.
In: Biofactors., Vol. 40, No. 6, 11.2014, p. 611-623.Research output: Contribution to journal › Article
TY - JOUR
T1 - Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health.
AU - Brown, Emma
AU - Nitecki, Sonja
AU - Pereira-Caro, Gemma
AU - Mc Dougall, Gordon
AU - Stewart, Derek
AU - Crozier, Alan
AU - Rowland, ian
AU - Gill, Chris IR
PY - 2014/11
Y1 - 2014/11
N2 - The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P <0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P <0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.
AB - The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P <0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P <0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.
KW - Polyphenols
KW - in vitro digestion
KW - ileal fluid
KW - diet
KW - gut health
KW - lingonberries
KW - DNA damage
KW - in vivo digestion
U2 - 10.1002/biof.1173
DO - 10.1002/biof.1173
M3 - Article
VL - 40
SP - 611
EP - 623
JO - BioFactors
T2 - BioFactors
JF - BioFactors
SN - 0951-6433
IS - 6
ER -