Comparative Reducing and Carbohydrate Enzyme Inhibitory Activities of the Root, Leaf, and Seed of Picralima nitida

Background: The ethnomedicinal uses of Picralima nitida is partly due to its antidiabetic properties.
Purpose: In this study, the antioxidant and total reducing power, as well as the carbohydrate hydrolysing enzymes’ inhibition potentials of the root, leaf, and seed of P. nitida were compared.
Methods: The antioxidant reducing power of P. nitida as well as its ability to inhibit selected enzymes of carbohydrate hydrolysis were evaluated using standard experimental methods.
Results: Methanol extract of P. nitida leaves exhibited the strongest reducing power (653.52 mgASC/g) followed
by the seed extract (620.11 mgASC/g). The free radical scavenging powers of the extracts were concentration
dependent. P. nitida seed extract exhibited the highest scavenging ability (IC50 = 190.7 µg/ml) as compared to
the root extract (IC50 = 470.40 µg/ml) and leaf extract (IC50 = 560.65 µg/ml). However, the seed extract had the
highest ABTS radical scavenging ability (IC50 = 174.02 ± 0.75 µg/ml) in comparison with the root extract (IC50
= 181.83 ± 1.01 µg/ml) and leaf extract (IC50 = 279.87 ± 1.39 µg/ml). Alpha-amylase and alpha-glucosidase
inhibitory properties of the extracts increased steadily with increasing concentration, as that of glibenclamide.
The methanolic extract of P. nitida seed had the highest inhibitory activity (95.70 %) as compared to that of the
root (92.39 %) and leaf (83.58 %) extracts.
Conclusion: P. nitida show strong reducing as well as enzyme inhibitory properties which are concentration
dependent and compares favourably with glibenclamide. Comparatively, the methanolic extract of the leaf of
P. nitida exhibited the highest reducing power and inhibition of α-amylase, while the methanolic seed extract
exhibited the highest ability to fight free radicals and inhibit the activity of α-glucosidase