TY - JOUR
T1 - Comparative in vitro evaluation of glimepiride containing nanosuspension drug delivery system developed by different techniques
AU - Bose, Sujit
AU - Sharma, Pooja
AU - Mishra, Vijay
AU - Patial, Swati
AU - Saraogi, Gaurav K.
AU - Tambuwala, Murtaza M.
AU - Dua, Kamal
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/1/11
Y1 - 2021/1/11
N2 - The present study was aimed to develop the Glimepiride (GLM) loaded nanosuspension by different methods for increasing the solubility of GLM. Twelve formulations were prepared by combination method (FG), which included antisolvent precipitation method followed by sonication (Method 1) selecting drug and polymer in ratio of 1:10, 1:20 and 1:30. Further 6 formulations were prepared by nanoprecipitation method (Fg) (Method 2) selecting drug and polymer in ratio of 1:10, 1:20 by using different polymers like polyvinyl pyrolidone (PVP K30), and poly(ethylene glycol) (PEG) such as PEG6000 and PEG400. The GLM nanosuspensions prepared by different techniques were evaluated by optical microscopy, percent entrapment efficiency (%EE), particle size analysis, zeta potential, transmission electron microscopy (TEM) and in vitro dissolution. FG1 formulation was found to be better formulation showing 82.04% EE, 129-180 nm particle size range, 30.16 mV zeta potential, 0.253 polydispersity index (PDI) and 86.76% drug release as compared to Fgii formulation having %EE, average particle size, zeta potential, PDI value and drug release as 80.03%, 72-383 nm, -22.19 mV, 0.358 and 74.77%, respectively. On the basis of results obtained from different studies, it can be concluded that GLM nanosuspension prepared by combination technique (FG) shows good solubility and dissolution than those prepared by nanoprecipitation technique (Fg), hence the combination technique is found to be a preferred technique to prepare GLM nanosuspension over nanoprecipitation technique.
AB - The present study was aimed to develop the Glimepiride (GLM) loaded nanosuspension by different methods for increasing the solubility of GLM. Twelve formulations were prepared by combination method (FG), which included antisolvent precipitation method followed by sonication (Method 1) selecting drug and polymer in ratio of 1:10, 1:20 and 1:30. Further 6 formulations were prepared by nanoprecipitation method (Fg) (Method 2) selecting drug and polymer in ratio of 1:10, 1:20 by using different polymers like polyvinyl pyrolidone (PVP K30), and poly(ethylene glycol) (PEG) such as PEG6000 and PEG400. The GLM nanosuspensions prepared by different techniques were evaluated by optical microscopy, percent entrapment efficiency (%EE), particle size analysis, zeta potential, transmission electron microscopy (TEM) and in vitro dissolution. FG1 formulation was found to be better formulation showing 82.04% EE, 129-180 nm particle size range, 30.16 mV zeta potential, 0.253 polydispersity index (PDI) and 86.76% drug release as compared to Fgii formulation having %EE, average particle size, zeta potential, PDI value and drug release as 80.03%, 72-383 nm, -22.19 mV, 0.358 and 74.77%, respectively. On the basis of results obtained from different studies, it can be concluded that GLM nanosuspension prepared by combination technique (FG) shows good solubility and dissolution than those prepared by nanoprecipitation technique (Fg), hence the combination technique is found to be a preferred technique to prepare GLM nanosuspension over nanoprecipitation technique.
KW - Antidiabetic
KW - Diabetes mellitus
KW - Drug delivery
KW - Glimepiride
KW - Nanosuspension
KW - Polymers
UR - http://www.scopus.com/inward/record.url?scp=85099628222&partnerID=8YFLogxK
U2 - 10.1016/j.molstruc.2021.129927
DO - 10.1016/j.molstruc.2021.129927
M3 - Article
VL - 1231
JO - Journal of Molecular Structure
JF - Journal of Molecular Structure
SN - 0022-2860
M1 - 129927
ER -