Comparative in vitro cytotoxicity study of carbon nanotubes and titania nanostructures on human lung epithelial cells

S Wadhwa, C Rea, P O'Hare, A Mathur, SS Roy, PSM Dunlop, JA Byrne, GA Burke, BJ Meenan, JAD McLaughlin

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Abstract

The aim of this study is to assess in vitro cytotoxic effects of titania nanostructures and carbon nanotubes (CNTs) by exposing A549 lung epithelial cell line to these materials. Titania nanotubes (TiNTs) were grown by hydrothermal treatment of TiO2 nanoparticles, followed by annealing them at 400 ◦C. The titania nanostructures obtained on annealing (mixture of nanotubes and nanorods) were hollow and open ended, containing 3–5 layers of titania sheets, with an internal diameter ∼3–5nm and external diameter ∼8–10 nm, and a specific surface area of 265m2/g. As-supplied single walled (SWCNTs) and microwave plasma enhanced chemical vapour deposition (MPCVD) grown multi walled carbon nanotubes (MWCNTs) were used in this study. The lengths and diameters of the SWCNTs were 5–10nm and 0.5–3nm respectively. The lengths and diameters of the MWCNTs were 25–30m and 10–30nm respectively. The cell viability was evaluated using the MTT (3-(4,-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium) assay. No significant cytotoxic effects of titania nanostructures were observed over a period of a week of testing time, while the presence of CNTs in some cases demonstrated significant cytotoxic effects. Finally, possible reason of cytotoxicity is discussed in the light of microstructures of materials. © 2011 Elsevier B.V. All rights reserved.
LanguageEnglish
Pages56-61
JournalJournal of Hazardous Materials
Volume191
DOIs
Publication statusPublished - 2011

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Carbon Nanotubes
Nanostructures
Cytotoxicity
titanium
Carbon nanotubes
Nanotubes
Titanium
Epithelial Cells
Lung
annealing
Titanium sheet
Annealing
Plasma enhanced chemical vapor deposition
Nanorods
Specific surface area
Assays
Microwaves
Nanoparticles
Cells
Cell Survival

Cite this

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title = "Comparative in vitro cytotoxicity study of carbon nanotubes and titania nanostructures on human lung epithelial cells",
abstract = "The aim of this study is to assess in vitro cytotoxic effects of titania nanostructures and carbon nanotubes (CNTs) by exposing A549 lung epithelial cell line to these materials. Titania nanotubes (TiNTs) were grown by hydrothermal treatment of TiO2 nanoparticles, followed by annealing them at 400 ◦C. The titania nanostructures obtained on annealing (mixture of nanotubes and nanorods) were hollow and open ended, containing 3–5 layers of titania sheets, with an internal diameter ∼3–5nm and external diameter ∼8–10 nm, and a specific surface area of 265m2/g. As-supplied single walled (SWCNTs) and microwave plasma enhanced chemical vapour deposition (MPCVD) grown multi walled carbon nanotubes (MWCNTs) were used in this study. The lengths and diameters of the SWCNTs were 5–10nm and 0.5–3nm respectively. The lengths and diameters of the MWCNTs were 25–30m and 10–30nm respectively. The cell viability was evaluated using the MTT (3-(4,-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium) assay. No significant cytotoxic effects of titania nanostructures were observed over a period of a week of testing time, while the presence of CNTs in some cases demonstrated significant cytotoxic effects. Finally, possible reason of cytotoxicity is discussed in the light of microstructures of materials. {\circledC} 2011 Elsevier B.V. All rights reserved.",
author = "S Wadhwa and C Rea and P O'Hare and A Mathur and SS Roy and PSM Dunlop and JA Byrne and GA Burke and BJ Meenan and JAD McLaughlin",
year = "2011",
doi = "10.1016/j.hazmat.2011.04.035",
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TY - JOUR

T1 - Comparative in vitro cytotoxicity study of carbon nanotubes and titania nanostructures on human lung epithelial cells

AU - Wadhwa, S

AU - Rea, C

AU - O'Hare, P

AU - Mathur, A

AU - Roy, SS

AU - Dunlop, PSM

AU - Byrne, JA

AU - Burke, GA

AU - Meenan, BJ

AU - McLaughlin, JAD

PY - 2011

Y1 - 2011

N2 - The aim of this study is to assess in vitro cytotoxic effects of titania nanostructures and carbon nanotubes (CNTs) by exposing A549 lung epithelial cell line to these materials. Titania nanotubes (TiNTs) were grown by hydrothermal treatment of TiO2 nanoparticles, followed by annealing them at 400 ◦C. The titania nanostructures obtained on annealing (mixture of nanotubes and nanorods) were hollow and open ended, containing 3–5 layers of titania sheets, with an internal diameter ∼3–5nm and external diameter ∼8–10 nm, and a specific surface area of 265m2/g. As-supplied single walled (SWCNTs) and microwave plasma enhanced chemical vapour deposition (MPCVD) grown multi walled carbon nanotubes (MWCNTs) were used in this study. The lengths and diameters of the SWCNTs were 5–10nm and 0.5–3nm respectively. The lengths and diameters of the MWCNTs were 25–30m and 10–30nm respectively. The cell viability was evaluated using the MTT (3-(4,-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium) assay. No significant cytotoxic effects of titania nanostructures were observed over a period of a week of testing time, while the presence of CNTs in some cases demonstrated significant cytotoxic effects. Finally, possible reason of cytotoxicity is discussed in the light of microstructures of materials. © 2011 Elsevier B.V. All rights reserved.

AB - The aim of this study is to assess in vitro cytotoxic effects of titania nanostructures and carbon nanotubes (CNTs) by exposing A549 lung epithelial cell line to these materials. Titania nanotubes (TiNTs) were grown by hydrothermal treatment of TiO2 nanoparticles, followed by annealing them at 400 ◦C. The titania nanostructures obtained on annealing (mixture of nanotubes and nanorods) were hollow and open ended, containing 3–5 layers of titania sheets, with an internal diameter ∼3–5nm and external diameter ∼8–10 nm, and a specific surface area of 265m2/g. As-supplied single walled (SWCNTs) and microwave plasma enhanced chemical vapour deposition (MPCVD) grown multi walled carbon nanotubes (MWCNTs) were used in this study. The lengths and diameters of the SWCNTs were 5–10nm and 0.5–3nm respectively. The lengths and diameters of the MWCNTs were 25–30m and 10–30nm respectively. The cell viability was evaluated using the MTT (3-(4,-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium) assay. No significant cytotoxic effects of titania nanostructures were observed over a period of a week of testing time, while the presence of CNTs in some cases demonstrated significant cytotoxic effects. Finally, possible reason of cytotoxicity is discussed in the light of microstructures of materials. © 2011 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.hazmat.2011.04.035

DO - 10.1016/j.hazmat.2011.04.035

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VL - 191

SP - 56

EP - 61

JO - Journal of Hazardous Materials

T2 - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

ER -