Comparative Genomics of Carriage and Disease Isolates of Streptococcus pneumoniae Serotype 22F Reveals Lineage-Specific Divergence and Niche Adaptation.

David W Cleary, Vanessa Devine, Johanna M C Jefferies, Jeremy S Webb, Stephen D Bentley, Rebecca A Gladstone, Saul N Faust, Stuart C Clarke

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Streptococcus pneumoniae is a major cause of meningitis, sepsis, and pneumonia worldwide. Pneumococcal conjugate vaccines have been part of the United Kingdom's childhood immunization program since 2006 and have significantly reduced the incidence of disease due to vaccine efficacy in reducing carriage in the population. Here we isolated two clones of 22F (an emerging serotype of clinical concern, multilocus sequence types 433 and 698) and conducted comparative genomic analysis on four isolates, paired by Sequence Type (ST) with one of each pair being derived from carriage and the other disease (sepsis). The most compelling observation was of nonsynonymous mutations in pgdA, encoding peptidoglycan N-acetylglucosamine deacetylase A, which was found in the carriage isolates of both ST433 and 698. Deacetylation of pneumococcal peptidoglycan is known to enable resistance to lysozyme upon invasion. Althought no other clear genotypic signatures related to disease or carriage could be determined, additional intriguing comparisons between the two STs were possible. These include the presence of an intact prophage, in addition to numerous additional phage insertions, within the carriage isolate of ST433. Contrasting gene repertoires related to virulence and colonization, including bacteriocins, lantibiotics, and toxin--antitoxin systems, were also observed.
LanguageEnglish
Pages1243-1251
Number of pages8
JournalGenome Biology and Evolution
Volume8
Issue number4
DOIs
Publication statusPublished - 24 Mar 2016

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Genomics
Streptococcus pneumoniae
Bacteriocins
N-acetylglucosamine deacetylase
Peptidoglycan
Sepsis
Antitoxins
Prophages
Immunization Programs
Conjugate Vaccines
Pneumococcal Vaccines
Muramidase
Meningitis
Bacteriophages
Virulence
Pneumonia
Vaccines
Clone Cells
Observation
Mutation

Keywords

  • Streptococcus pneumoniae
  • genome sequencing
  • invasive pneumococcal disease

Cite this

Cleary, David W ; Devine, Vanessa ; Jefferies, Johanna M C ; Webb, Jeremy S ; Bentley, Stephen D ; Gladstone, Rebecca A ; Faust, Saul N ; Clarke, Stuart C. / Comparative Genomics of Carriage and Disease Isolates of Streptococcus pneumoniae Serotype 22F Reveals Lineage-Specific Divergence and Niche Adaptation. In: Genome Biology and Evolution. 2016 ; Vol. 8, No. 4. pp. 1243-1251.
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abstract = "Streptococcus pneumoniae is a major cause of meningitis, sepsis, and pneumonia worldwide. Pneumococcal conjugate vaccines have been part of the United Kingdom's childhood immunization program since 2006 and have significantly reduced the incidence of disease due to vaccine efficacy in reducing carriage in the population. Here we isolated two clones of 22F (an emerging serotype of clinical concern, multilocus sequence types 433 and 698) and conducted comparative genomic analysis on four isolates, paired by Sequence Type (ST) with one of each pair being derived from carriage and the other disease (sepsis). The most compelling observation was of nonsynonymous mutations in pgdA, encoding peptidoglycan N-acetylglucosamine deacetylase A, which was found in the carriage isolates of both ST433 and 698. Deacetylation of pneumococcal peptidoglycan is known to enable resistance to lysozyme upon invasion. Althought no other clear genotypic signatures related to disease or carriage could be determined, additional intriguing comparisons between the two STs were possible. These include the presence of an intact prophage, in addition to numerous additional phage insertions, within the carriage isolate of ST433. Contrasting gene repertoires related to virulence and colonization, including bacteriocins, lantibiotics, and toxin--antitoxin systems, were also observed.",
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Comparative Genomics of Carriage and Disease Isolates of Streptococcus pneumoniae Serotype 22F Reveals Lineage-Specific Divergence and Niche Adaptation. / Cleary, David W; Devine, Vanessa; Jefferies, Johanna M C; Webb, Jeremy S; Bentley, Stephen D; Gladstone, Rebecca A; Faust, Saul N; Clarke, Stuart C.

In: Genome Biology and Evolution, Vol. 8, No. 4, 24.03.2016, p. 1243-1251.

Research output: Contribution to journalArticle

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