Comparasion of five gene loci (rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ) to aid the molecular identification of viridans-group streptococci and pneumococci

Y. Maeda, C. E. Goldsmith, W. A. Coulter, C. Mason, James Dooley, Colm Lowery, B. C. Millar, J. E. Moore

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Abstract

Viridans-group streptococci (VGS) consist of several taxa which historically have been highly diverse. However, at times it may become necessary to have a reliable scheme for the identification of these organisms to the species level. The aim of this study is to compare the ability of five gene loci, namely rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ, to speciate such organisms through a sequence typing-based approach. Reference organisms consisting of six VGS species were compared based on sequence typing, followed by comparison of 31 wild-type respiratory isolates, and showed that employment of sequence typing using the rnpB gene locus was the most specific and reliable. Therefore, the use of rnpB sequencing for the identification of VGS to species level is a reliable and feasible option, based on a single gene target.
LanguageEnglish
Pages190-196
JournalBritish Journal of Biomedical Science
Volume68
Issue number4
Publication statusPublished - 2011

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Streptococcus
ribosomal RNA
loci
organisms
genes

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Maeda, Y. ; Goldsmith, C. E. ; Coulter, W. A. ; Mason, C. ; Dooley, James ; Lowery, Colm ; Millar, B. C. ; Moore, J. E. / Comparasion of five gene loci (rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ) to aid the molecular identification of viridans-group streptococci and pneumococci. 2011 ; Vol. 68, No. 4. pp. 190-196.
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Comparasion of five gene loci (rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ) to aid the molecular identification of viridans-group streptococci and pneumococci. / Maeda, Y.; Goldsmith, C. E.; Coulter, W. A.; Mason, C.; Dooley, James; Lowery, Colm; Millar, B. C.; Moore, J. E.

Vol. 68, No. 4, 2011, p. 190-196.

Research output: Contribution to journalArticle

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AU - Moore, J. E.

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AB - Viridans-group streptococci (VGS) consist of several taxa which historically have been highly diverse. However, at times it may become necessary to have a reliable scheme for the identification of these organisms to the species level. The aim of this study is to compare the ability of five gene loci, namely rnpB, 16S rRNA, 16S-23S rRNA, sodA and dnaJ, to speciate such organisms through a sequence typing-based approach. Reference organisms consisting of six VGS species were compared based on sequence typing, followed by comparison of 31 wild-type respiratory isolates, and showed that employment of sequence typing using the rnpB gene locus was the most specific and reliable. Therefore, the use of rnpB sequencing for the identification of VGS to species level is a reliable and feasible option, based on a single gene target.

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