Colon-available raspberry polyphenols exhibit anti-cancer effects on in vitro models of colon cancer

E.M Brown, G Popa, C.I.R Gill, M.J McCann, G.J McDougall, D Stewart, I Rowland

    Research output: Contribution to journalArticle

    96 Citations (Scopus)

    Abstract

    BACKGROUND:There is a probable association between consumption of fruit and vegetables and reduced risk of cancer, particularly cancer of the digestive tract. This anti-cancer activity has been attributed in part to anti-oxidants present in these foods. Raspberries in particular are a rich source of the anti-oxidant compounds, such as polyphenols, anthocyanins and ellagitannins.METHODS:A "colon-available" raspberry extract (CARE) was prepared that contained phytochemicals surviving a digestion procedure that mimicked the physiochemical conditions of the upper gastrointestinal tract. The polyphenolic-rich extract was assessed for anti-cancer properties in a series of in vitro systems that model important stages of colon carcinogenesis, initiation, promotion and invasion.RESULTS:The phytochemical composition of CARE was monitored using liquid chromatography mass spectrometry. The colon-available raspberry extract was reduced in anthocyanins and ellagitannins compared to the original raspberry juice but enriched in other polyphenols and polyphenol breakdown products that were more stable to gastrointestinal digestion. Initiation--CARE caused significant protective effects against DNA damage induced by hydrogen peroxide in HT29 colon cancer cells measured using single cell microgelelectrophoresis. Promotion--CARE significantly decreased the population of HT29 cells in the G1 phase of the cell cycle, effectively reducing the number of cells entering the cell cycle. However, CARE had no effect on epithelial integrity (barrier function) assessed by recording the trans-epithelial resistance (TER) of CACO-2 cell monolayers. Invasion--CARE caused significant inhibition of HT115 colon cancer cell invasion using the matrigel invasion assay.CONCLUSION:The results indicate that raspberry phytochemicals likely to reach the colon are capable of inhibiting several important stages in colon carcinogenesis in vitro.
    LanguageEnglish
    Pages4-13
    JournalJournal of Carcinogenesis
    Volume6
    DOIs
    Publication statusPublished - 18 Apr 2007

    Fingerprint

    Polyphenols
    Colonic Neoplasms
    Colon
    Neoplasms
    Phytochemicals
    Hydrolyzable Tannins
    Anthocyanins
    Oxidants
    Digestion
    Cell Cycle
    Carcinogenesis
    Rubus
    In Vitro Techniques
    HT29 Cells
    Upper Gastrointestinal Tract
    G1 Phase
    Liquid Chromatography
    Vegetables
    Hydrogen Peroxide
    DNA Damage

    Cite this

    Brown, E.M ; Popa, G ; Gill, C.I.R ; McCann, M.J ; McDougall, G.J ; Stewart, D ; Rowland, I. / Colon-available raspberry polyphenols exhibit anti-cancer effects on in vitro models of colon cancer. In: Journal of Carcinogenesis. 2007 ; Vol. 6. pp. 4-13.
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    abstract = "BACKGROUND:There is a probable association between consumption of fruit and vegetables and reduced risk of cancer, particularly cancer of the digestive tract. This anti-cancer activity has been attributed in part to anti-oxidants present in these foods. Raspberries in particular are a rich source of the anti-oxidant compounds, such as polyphenols, anthocyanins and ellagitannins.METHODS:A {"}colon-available{"} raspberry extract (CARE) was prepared that contained phytochemicals surviving a digestion procedure that mimicked the physiochemical conditions of the upper gastrointestinal tract. The polyphenolic-rich extract was assessed for anti-cancer properties in a series of in vitro systems that model important stages of colon carcinogenesis, initiation, promotion and invasion.RESULTS:The phytochemical composition of CARE was monitored using liquid chromatography mass spectrometry. The colon-available raspberry extract was reduced in anthocyanins and ellagitannins compared to the original raspberry juice but enriched in other polyphenols and polyphenol breakdown products that were more stable to gastrointestinal digestion. Initiation--CARE caused significant protective effects against DNA damage induced by hydrogen peroxide in HT29 colon cancer cells measured using single cell microgelelectrophoresis. Promotion--CARE significantly decreased the population of HT29 cells in the G1 phase of the cell cycle, effectively reducing the number of cells entering the cell cycle. However, CARE had no effect on epithelial integrity (barrier function) assessed by recording the trans-epithelial resistance (TER) of CACO-2 cell monolayers. Invasion--CARE caused significant inhibition of HT115 colon cancer cell invasion using the matrigel invasion assay.CONCLUSION:The results indicate that raspberry phytochemicals likely to reach the colon are capable of inhibiting several important stages in colon carcinogenesis in vitro.",
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    doi = "10.1186/1477-3163-6-4",
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    Colon-available raspberry polyphenols exhibit anti-cancer effects on in vitro models of colon cancer. / Brown, E.M; Popa, G; Gill, C.I.R; McCann, M.J; McDougall, G.J; Stewart, D; Rowland, I.

    In: Journal of Carcinogenesis, Vol. 6, 18.04.2007, p. 4-13.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Colon-available raspberry polyphenols exhibit anti-cancer effects on in vitro models of colon cancer

    AU - Brown, E.M

    AU - Popa, G

    AU - Gill, C.I.R

    AU - McCann, M.J

    AU - McDougall, G.J

    AU - Stewart, D

    AU - Rowland, I

    PY - 2007/4/18

    Y1 - 2007/4/18

    N2 - BACKGROUND:There is a probable association between consumption of fruit and vegetables and reduced risk of cancer, particularly cancer of the digestive tract. This anti-cancer activity has been attributed in part to anti-oxidants present in these foods. Raspberries in particular are a rich source of the anti-oxidant compounds, such as polyphenols, anthocyanins and ellagitannins.METHODS:A "colon-available" raspberry extract (CARE) was prepared that contained phytochemicals surviving a digestion procedure that mimicked the physiochemical conditions of the upper gastrointestinal tract. The polyphenolic-rich extract was assessed for anti-cancer properties in a series of in vitro systems that model important stages of colon carcinogenesis, initiation, promotion and invasion.RESULTS:The phytochemical composition of CARE was monitored using liquid chromatography mass spectrometry. The colon-available raspberry extract was reduced in anthocyanins and ellagitannins compared to the original raspberry juice but enriched in other polyphenols and polyphenol breakdown products that were more stable to gastrointestinal digestion. Initiation--CARE caused significant protective effects against DNA damage induced by hydrogen peroxide in HT29 colon cancer cells measured using single cell microgelelectrophoresis. Promotion--CARE significantly decreased the population of HT29 cells in the G1 phase of the cell cycle, effectively reducing the number of cells entering the cell cycle. However, CARE had no effect on epithelial integrity (barrier function) assessed by recording the trans-epithelial resistance (TER) of CACO-2 cell monolayers. Invasion--CARE caused significant inhibition of HT115 colon cancer cell invasion using the matrigel invasion assay.CONCLUSION:The results indicate that raspberry phytochemicals likely to reach the colon are capable of inhibiting several important stages in colon carcinogenesis in vitro.

    AB - BACKGROUND:There is a probable association between consumption of fruit and vegetables and reduced risk of cancer, particularly cancer of the digestive tract. This anti-cancer activity has been attributed in part to anti-oxidants present in these foods. Raspberries in particular are a rich source of the anti-oxidant compounds, such as polyphenols, anthocyanins and ellagitannins.METHODS:A "colon-available" raspberry extract (CARE) was prepared that contained phytochemicals surviving a digestion procedure that mimicked the physiochemical conditions of the upper gastrointestinal tract. The polyphenolic-rich extract was assessed for anti-cancer properties in a series of in vitro systems that model important stages of colon carcinogenesis, initiation, promotion and invasion.RESULTS:The phytochemical composition of CARE was monitored using liquid chromatography mass spectrometry. The colon-available raspberry extract was reduced in anthocyanins and ellagitannins compared to the original raspberry juice but enriched in other polyphenols and polyphenol breakdown products that were more stable to gastrointestinal digestion. Initiation--CARE caused significant protective effects against DNA damage induced by hydrogen peroxide in HT29 colon cancer cells measured using single cell microgelelectrophoresis. Promotion--CARE significantly decreased the population of HT29 cells in the G1 phase of the cell cycle, effectively reducing the number of cells entering the cell cycle. However, CARE had no effect on epithelial integrity (barrier function) assessed by recording the trans-epithelial resistance (TER) of CACO-2 cell monolayers. Invasion--CARE caused significant inhibition of HT115 colon cancer cell invasion using the matrigel invasion assay.CONCLUSION:The results indicate that raspberry phytochemicals likely to reach the colon are capable of inhibiting several important stages in colon carcinogenesis in vitro.

    U2 - 10.1186/1477-3163-6-4

    DO - 10.1186/1477-3163-6-4

    M3 - Article

    VL - 6

    SP - 4

    EP - 13

    JO - Journal of Carcinogenesis

    T2 - Journal of Carcinogenesis

    JF - Journal of Carcinogenesis

    SN - 0974-6773

    ER -