Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons.

Hongjuan Wang, Rong Wang, Zhiwei Zhao, Zhijuan Ji, Shiming Xu, Christian Holscher, Shuli Sheng

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Type 2 diabetes recently has been identified as a risk factor for developing Alzheimer's disease (AD). The main reason for this appears to be insulin signaling failure in the brain. Furthermore, cholinergic neurons are particularly affected in the brains of AD patients. The aim of the present study is to investigate if insulin signaling-related proteins are co-located with cholinergic neuron in the CA1 region of hippocampus of mice, which could explain the early loss of cholinergic neurons in AD. Using immunohistochemistry, the insulin signaling-related proteins, such as insulin receptor (InsR), insulin receptor substrate-1 (IRS-1), protein kinase B (PKB, also named Akt), glycogen synthase kinase-3beta (GSK-3beta) and insulin-degrading enzyme (IDE) were analysed. Choline acetyltransferase (ChAT) was selected as a marker of cholinergic neurons. In the CA1 region of hippocampus of mice, several of the insulin signaling-related proteins we had chosen are co-located with ChAT, and most double immunoreactive positive cells were pyramidal cells. The coexistences indicated that the insulin signaling may play an important part in the activities of cholinergic neurons, and the impairment of the pathway may be important in the mechanisms that underlie neurodegeneration in AD.
    LanguageEnglish
    Pages237-43
    JournalBRAIN RESEARCH
    Volume1249
    Publication statusPublished - 2009

    Fingerprint

    Cholinergic Neurons
    Choline O-Acetyltransferase
    Insulin
    Alzheimer Disease
    Neurons
    Proteins
    Hippocampus
    Insulysin
    Glycogen Synthase Kinases
    Insulin Receptor Substrate Proteins
    Proto-Oncogene Proteins c-akt
    Pyramidal Cells
    Insulin Receptor
    Brain Diseases
    Type 2 Diabetes Mellitus
    Immunohistochemistry
    Brain

    Cite this

    Wang, H., Wang, R., Zhao, Z., Ji, Z., Xu, S., Holscher, C., & Sheng, S. (2009). Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons. 1249, 237-43.
    Wang, Hongjuan ; Wang, Rong ; Zhao, Zhiwei ; Ji, Zhijuan ; Xu, Shiming ; Holscher, Christian ; Sheng, Shuli. / Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons. 2009 ; Vol. 1249. pp. 237-43.
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    abstract = "Type 2 diabetes recently has been identified as a risk factor for developing Alzheimer's disease (AD). The main reason for this appears to be insulin signaling failure in the brain. Furthermore, cholinergic neurons are particularly affected in the brains of AD patients. The aim of the present study is to investigate if insulin signaling-related proteins are co-located with cholinergic neuron in the CA1 region of hippocampus of mice, which could explain the early loss of cholinergic neurons in AD. Using immunohistochemistry, the insulin signaling-related proteins, such as insulin receptor (InsR), insulin receptor substrate-1 (IRS-1), protein kinase B (PKB, also named Akt), glycogen synthase kinase-3beta (GSK-3beta) and insulin-degrading enzyme (IDE) were analysed. Choline acetyltransferase (ChAT) was selected as a marker of cholinergic neurons. In the CA1 region of hippocampus of mice, several of the insulin signaling-related proteins we had chosen are co-located with ChAT, and most double immunoreactive positive cells were pyramidal cells. The coexistences indicated that the insulin signaling may play an important part in the activities of cholinergic neurons, and the impairment of the pathway may be important in the mechanisms that underlie neurodegeneration in AD.",
    author = "Hongjuan Wang and Rong Wang and Zhiwei Zhao and Zhijuan Ji and Shiming Xu and Christian Holscher and Shuli Sheng",
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    Wang, H, Wang, R, Zhao, Z, Ji, Z, Xu, S, Holscher, C & Sheng, S 2009, 'Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons.', vol. 1249, pp. 237-43.

    Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons. / Wang, Hongjuan; Wang, Rong; Zhao, Zhiwei; Ji, Zhijuan; Xu, Shiming; Holscher, Christian; Sheng, Shuli.

    Vol. 1249, 2009, p. 237-43.

    Research output: Contribution to journalArticle

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    AU - Holscher, Christian

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    AB - Type 2 diabetes recently has been identified as a risk factor for developing Alzheimer's disease (AD). The main reason for this appears to be insulin signaling failure in the brain. Furthermore, cholinergic neurons are particularly affected in the brains of AD patients. The aim of the present study is to investigate if insulin signaling-related proteins are co-located with cholinergic neuron in the CA1 region of hippocampus of mice, which could explain the early loss of cholinergic neurons in AD. Using immunohistochemistry, the insulin signaling-related proteins, such as insulin receptor (InsR), insulin receptor substrate-1 (IRS-1), protein kinase B (PKB, also named Akt), glycogen synthase kinase-3beta (GSK-3beta) and insulin-degrading enzyme (IDE) were analysed. Choline acetyltransferase (ChAT) was selected as a marker of cholinergic neurons. In the CA1 region of hippocampus of mice, several of the insulin signaling-related proteins we had chosen are co-located with ChAT, and most double immunoreactive positive cells were pyramidal cells. The coexistences indicated that the insulin signaling may play an important part in the activities of cholinergic neurons, and the impairment of the pathway may be important in the mechanisms that underlie neurodegeneration in AD.

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    Wang H, Wang R, Zhao Z, Ji Z, Xu S, Holscher C et al. Coexistences of insulin signaling-related proteins and choline acetyltransferase in neurons. 2009;1249:237-43.