Cloning of maximakinin precursor cDNAs from Chinese toad, Bombina maxima, venom

TB Chen, AJ Bjourson, Stephen McClean, DF Orr, EJ O'Kane, PF Rao, C Shaw

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


Using a novel technique that we have developed for cloning of amphibian skin secretion peptide cDNAs from lyophilized samples, we report here that maximakinin (DLPKINRKGP-bradykinin) is encoded by two different cDNAs, named BMK-1 and BMK-2, containing either four tandem repeat sequences or a single copy. The open reading frames of both precursor cDNAs were found to be 152 and 116 amino acid residues, respectively. These data provide evidence that the structural diversity of peptides in amphibian skin secretions arising front molecular evolutionary events, can be mediated by parallel diversity in encoding mRNAs that in itself may reflect serial gene duplications. (C) 2003 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)853-861
Issue number6
Publication statusPublished (in print/issue) - Jun 2003


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