Chemical and immunochemical characterisation of substance P-like immunoreactivity and physalaemin-like immunoreactivity in a carcinoid tumour

J. M. Conlon, G. Schäfer, W. E. Schmidt, L. H. Lazarus, H. D. Becker, W. Creutzfeldt

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11 Citations (Scopus)

Abstract

Extracts of a carcinoid tumour, resected from the mid-portion of the ileum of a patient with no symptoms of endocrine disorder, were associated with a high concentration of substance P-like immunoreactivity. Using reverse-phase high performance liquid chromatography and antisera specifically directed against the C-terminal and N-terminal regions of substance P and against the N-terminal region of physalaemin, the following components were isolated and identified: substance P and its oxidised form, [pGlu5]substance P-(5-11) peptide and its oxidised form, and the oxidised form of physalaemin. The identity of tumour substance P with the undecapeptide was confirmed by amino acid analysis and high performance ion-exchange chromatography. In vitro incubation of tumour tissue from a lymph node metastasis from the same patient with [3H]leucine resulted in incorporation of radioactivity into newly synthesised substance P.

Original languageEnglish
Pages (from-to)117-132
Number of pages16
JournalRegulatory Peptides
Volume11
Issue number2
DOIs
Publication statusPublished (in print/issue) - Jun 1985

Keywords

  • biosynthesis
  • HPLC
  • neurokinin
  • radioimmunoassay
  • tachykinin

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