TY - JOUR
T1 - Characterization of trout galanin and its distribution in trout brain and pituitary
AU - Anglade, Isabelle
AU - Wang, Yunxia
AU - Jensen, Jorgen
AU - Tramu, Gerard
AU - Kah, Olivier
AU - Conlon, J. Michael
PY - 1994/12/1
Y1 - 1994/12/1
N2 - Galanin was purified from an extract of the stomach of the rainbow trout, Oncorhynchus mykiss, and its primary structure was established as Gly‐Trp‐Thr‐Leu‐Asn‐Ser‐Ala‐Gly‐Tyr‐Leu10‐Leu‐Gly‐Pro‐His‐Gly‐Ile‐Asp‐Gly‐His‐Arg20‐Thr‐Leu‐Ser‐Asp‐Lys‐His‐Gly‐Leu‐Ala. Trout galanin shows six amino acid substitutions compared with pig galanin, but the N‐terminal region (residues 1–14) has been fully conserved. The distribution of galanin‐immunoreactive (GAL‐IR) structures in the trout brain and pituitary was studied via immunohistochemistry. GAL‐IR cell bodies were observed only in the caudal telencephalon, the preoptic region, and the mediobasal hypothalamus. GAL‐IR fibers, however, are widely distributed throughout the brain, with a much lower density in the midbrain and posterior brain than in the tel‐ and diencephalon. Particularly dense innervation of the mediobasal hypothalamus, the ventral and supracommissuralis parts of the caudal telencephalon, and the region above and below the anterior commissure was observed. A heavy innervation of the pituitary was consistently detected. GAL‐IR fibers were present in neurohypophyseal digitations of both the anterior and intermediate lobes with highest density in the region of the proximal pars distalis, where growth hormone and gonadotropic cells are located. Fibers were also seen in digitations of the rostral pars distalis, in particular between the prolactin follicles. The distribution of GAL‐IR neurons in the central nervous system and pituitary of the trout suggests that the peptide may exercise an important role in the regulation of neuroendocrine functions, particularly those related to reproduction. © 1994 Wiley‐Liss, Inc.
AB - Galanin was purified from an extract of the stomach of the rainbow trout, Oncorhynchus mykiss, and its primary structure was established as Gly‐Trp‐Thr‐Leu‐Asn‐Ser‐Ala‐Gly‐Tyr‐Leu10‐Leu‐Gly‐Pro‐His‐Gly‐Ile‐Asp‐Gly‐His‐Arg20‐Thr‐Leu‐Ser‐Asp‐Lys‐His‐Gly‐Leu‐Ala. Trout galanin shows six amino acid substitutions compared with pig galanin, but the N‐terminal region (residues 1–14) has been fully conserved. The distribution of galanin‐immunoreactive (GAL‐IR) structures in the trout brain and pituitary was studied via immunohistochemistry. GAL‐IR cell bodies were observed only in the caudal telencephalon, the preoptic region, and the mediobasal hypothalamus. GAL‐IR fibers, however, are widely distributed throughout the brain, with a much lower density in the midbrain and posterior brain than in the tel‐ and diencephalon. Particularly dense innervation of the mediobasal hypothalamus, the ventral and supracommissuralis parts of the caudal telencephalon, and the region above and below the anterior commissure was observed. A heavy innervation of the pituitary was consistently detected. GAL‐IR fibers were present in neurohypophyseal digitations of both the anterior and intermediate lobes with highest density in the region of the proximal pars distalis, where growth hormone and gonadotropic cells are located. Fibers were also seen in digitations of the rostral pars distalis, in particular between the prolactin follicles. The distribution of GAL‐IR neurons in the central nervous system and pituitary of the trout suggests that the peptide may exercise an important role in the regulation of neuroendocrine functions, particularly those related to reproduction. © 1994 Wiley‐Liss, Inc.
KW - gonadotroph
KW - neuropeptide
KW - purification radioimmunoassay
KW - teleost
UR - http://www.scopus.com/inward/record.url?scp=0027940257&partnerID=8YFLogxK
U2 - 10.1002/cne.903500105
DO - 10.1002/cne.903500105
M3 - Article
C2 - 7532194
AN - SCOPUS:0027940257
SN - 0021-9967
VL - 350
SP - 63
EP - 74
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 1
ER -