Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils

Zulfiqar A. Raza; Zafar M. Khalid; Ibrahim Banat

doi:10.1080/10934520903217138

Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils

Zulfiqar A. Raza, Zafar M. Khalid, Ibrahim Banat

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Abstract

Pseudomonas aeruginosa EBN-8 mutant rhamnolipids produced on waste oils were investigated using normal-phase thin layer chromatography and fast atom bombardment mass spectrometry. Negative ion mode mass spectra yielded [M - H](-) ions and their fragment ions, which gave some indications on the sequence of rhamnolipid biosynthesis. Five rhamnolipid homologs [viz. RC10C10 (m/z 503), RC12C10 or RC10C12 (531), RRC10C8 or RRC8C10 (621), RRC10C10 (649) and RRC12C10 or RRC10C12 (677)] were detected in four rhamnolipid combinations under the different carbon sources. The prevalence of rhamnolipids was confirmed by Fourier transform infrared and one-dimensional proton nuclear magnetic resonance. We also observed some correlations between the tensioactive characteristics and structural chemistry of the rhamnolipid surfactants.

Original language	English
Pages (from-to)	1367-1373
Journal	Journal of Environmental Science and Health Part A
Volume	44
Issue number	13
DOIs	https://doi.org/10.1080/10934520903217138
Publication status	Published (in print/issue) - 2009

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title = "Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils",

abstract = "Pseudomonas aeruginosa EBN-8 mutant rhamnolipids produced on waste oils were investigated using normal-phase thin layer chromatography and fast atom bombardment mass spectrometry. Negative ion mode mass spectra yielded [M - H](-) ions and their fragment ions, which gave some indications on the sequence of rhamnolipid biosynthesis. Five rhamnolipid homologs [viz. RC10C10 (m/z 503), RC12C10 or RC10C12 (531), RRC10C8 or RRC8C10 (621), RRC10C10 (649) and RRC12C10 or RRC10C12 (677)] were detected in four rhamnolipid combinations under the different carbon sources. The prevalence of rhamnolipids was confirmed by Fourier transform infrared and one-dimensional proton nuclear magnetic resonance. We also observed some correlations between the tensioactive characteristics and structural chemistry of the rhamnolipid surfactants.",

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T1 - Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils

AU - Raza, Zulfiqar A.

AU - Khalid, Zafar M.

AU - Banat, Ibrahim

PY - 2009

Y1 - 2009

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AB - Pseudomonas aeruginosa EBN-8 mutant rhamnolipids produced on waste oils were investigated using normal-phase thin layer chromatography and fast atom bombardment mass spectrometry. Negative ion mode mass spectra yielded [M - H](-) ions and their fragment ions, which gave some indications on the sequence of rhamnolipid biosynthesis. Five rhamnolipid homologs [viz. RC10C10 (m/z 503), RC12C10 or RC10C12 (531), RRC10C8 or RRC8C10 (621), RRC10C10 (649) and RRC12C10 or RRC10C12 (677)] were detected in four rhamnolipid combinations under the different carbon sources. The prevalence of rhamnolipids was confirmed by Fourier transform infrared and one-dimensional proton nuclear magnetic resonance. We also observed some correlations between the tensioactive characteristics and structural chemistry of the rhamnolipid surfactants.

U2 - 10.1080/10934520903217138

DO - 10.1080/10934520903217138

M3 - Article

SN - 1532-4117

VL - 44

SP - 1367

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JO - Journal of Environmental Science and Health Part A

JF - Journal of Environmental Science and Health Part A

IS - 13

ER -

Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils

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