Characterization of an amidated form of pancreatic polypeptide from the daddy sculpin (Cottus scorpius)

J. M. Conlon; W. E. Schmidt; B. Gallwitz; S. Falkmer; L. Thim

doi:10.1016/0167-0115(86)90025-X

Characterization of an amidated form of pancreatic polypeptide from the daddy sculpin (Cottus scorpius)

J. M. Conlon
, W. E. Schmidt
, B. Gallwitz
, S. Falkmer
, L. Thim

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

The primary structure of pancreatic polypeptide from the teleostean fish, Cottus scorpius (daddy sculpin) was established as: Y P P Q P E S P G G N A S P E D W A K Y H A A V R H Y V N L I T R Q R Y NH₂. The presence of a COOH-terminally α-amidated amino acid was established using an HPLC method of general applicability. Although the peptide shows strong homology towards anglerfish pancreatic polypeptide (86%), homology towards porcine peptide YY (PYY) (61%) and porcine neuropeptide Y (NPY) (61%) was greater than towards porcine pancreatic polypeptide (PP) (47%). This result supports suggestions that the gene duplication events which led to PP, NPY and PYY formation took place after the time of divergence of fish and mammals.

Original language	English
Pages (from-to)	261-268
Number of pages	8
Journal	Regulatory Peptides
Volume	16
Issue number	3-4
DOIs	https://doi.org/10.1016/0167-0115(86)90025-X
Publication status	Published (in print/issue) - 30 Dec 1986

Keywords

HPLC
primary structure
teleost pancreatic islet

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10.1016/0167-0115(86)90025-X

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title = "Characterization of an amidated form of pancreatic polypeptide from the daddy sculpin (Cottus scorpius)",

abstract = "The primary structure of pancreatic polypeptide from the teleostean fish, Cottus scorpius (daddy sculpin) was established as: Y P P Q P E S P G G N A S P E D W A K Y H A A V R H Y V N L I T R Q R Y NH2. The presence of a COOH-terminally α-amidated amino acid was established using an HPLC method of general applicability. Although the peptide shows strong homology towards anglerfish pancreatic polypeptide (86\%), homology towards porcine peptide YY (PYY) (61\%) and porcine neuropeptide Y (NPY) (61\%) was greater than towards porcine pancreatic polypeptide (PP) (47\%). This result supports suggestions that the gene duplication events which led to PP, NPY and PYY formation took place after the time of divergence of fish and mammals.",

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doi = "10.1016/0167-0115(86)90025-X",

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T1 - Characterization of an amidated form of pancreatic polypeptide from the daddy sculpin (Cottus scorpius)

AU - Conlon, J. M.

AU - Schmidt, W. E.

AU - Gallwitz, B.

AU - Falkmer, S.

AU - Thim, L.

PY - 1986/12/30

Y1 - 1986/12/30

N2 - The primary structure of pancreatic polypeptide from the teleostean fish, Cottus scorpius (daddy sculpin) was established as: Y P P Q P E S P G G N A S P E D W A K Y H A A V R H Y V N L I T R Q R Y NH2. The presence of a COOH-terminally α-amidated amino acid was established using an HPLC method of general applicability. Although the peptide shows strong homology towards anglerfish pancreatic polypeptide (86%), homology towards porcine peptide YY (PYY) (61%) and porcine neuropeptide Y (NPY) (61%) was greater than towards porcine pancreatic polypeptide (PP) (47%). This result supports suggestions that the gene duplication events which led to PP, NPY and PYY formation took place after the time of divergence of fish and mammals.

AB - The primary structure of pancreatic polypeptide from the teleostean fish, Cottus scorpius (daddy sculpin) was established as: Y P P Q P E S P G G N A S P E D W A K Y H A A V R H Y V N L I T R Q R Y NH2. The presence of a COOH-terminally α-amidated amino acid was established using an HPLC method of general applicability. Although the peptide shows strong homology towards anglerfish pancreatic polypeptide (86%), homology towards porcine peptide YY (PYY) (61%) and porcine neuropeptide Y (NPY) (61%) was greater than towards porcine pancreatic polypeptide (PP) (47%). This result supports suggestions that the gene duplication events which led to PP, NPY and PYY formation took place after the time of divergence of fish and mammals.

KW - HPLC

KW - primary structure

KW - teleost pancreatic islet

UR - https://www.scopus.com/pages/publications/0022885393

U2 - 10.1016/0167-0115(86)90025-X

DO - 10.1016/0167-0115(86)90025-X

M3 - Article

C2 - 3562898

AN - SCOPUS:0022885393

SN - 0167-0115

VL - 16

SP - 261

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JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 3-4

ER -

Characterization of an amidated form of pancreatic polypeptide from the daddy sculpin (Cottus scorpius)

Abstract

Keywords

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