TY - JOUR
T1 - Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair
AU - Miyaji, EN
AU - Johnson, RT
AU - Downes, Stephen
AU - Eveno, E
AU - Mezzina, M
AU - Sarasin, A
AU - Menck, CFM
PY - 2000/9
Y1 - 2000/9
N2 - Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.
AB - Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.
M3 - Article
SN - 1678-4685
VL - 23
SP - 689
EP - 694
JO - Genetics and Molecular Biology
JF - Genetics and Molecular Biology
IS - 3
ER -