Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair

EN Miyaji, RT Johnson, Stephen Downes, E Eveno, M Mezzina, A Sarasin, CFM Menck

    Research output: Contribution to journalArticle

    Abstract

    Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.
    Original languageEnglish
    Pages (from-to)689-694
    JournalGenetics and Molecular Biology
    Volume23
    Issue number3
    Publication statusPublished - Sep 2000

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    Miyaji, EN., Johnson, RT., Downes, S., Eveno, E., Mezzina, M., Sarasin, A., & Menck, CFM. (2000). Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair. Genetics and Molecular Biology, 23(3), 689-694.