Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.
|Journal||Genetics and Molecular Biology|
|Publication status||Published - Sep 2000|
Miyaji, EN., Johnson, RT., Downes, S., Eveno, E., Mezzina, M., Sarasin, A., & Menck, CFM. (2000). Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair. Genetics and Molecular Biology, 23(3), 689-694.