Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair

EN Miyaji, RT Johnson, Stephen Downes, E Eveno, M Mezzina, A Sarasin, CFM Menck

    Research output: Contribution to journalArticle

    Abstract

    Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.
    LanguageEnglish
    Pages689-694
    JournalGenetics and Molecular Biology
    Volume23
    Issue number3
    Publication statusPublished - Sep 2000

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    Potoroidae
    DNA Repair
    Cell Cycle
    Clone Cells
    Pyrimidinones
    Pyrimidine Dimers
    Cell Line
    Ultraviolet Rays
    Cell Cycle Checkpoints
    DNA Replication
    S Phase
    Cell Death
    Cell Proliferation
    Phenotype
    DNA
    Genes

    Cite this

    Miyaji, EN., Johnson, RT., Downes, S., Eveno, E., Mezzina, M., Sarasin, A., & Menck, CFM. (2000). Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair. Genetics and Molecular Biology, 23(3), 689-694.
    Miyaji, EN ; Johnson, RT ; Downes, Stephen ; Eveno, E ; Mezzina, M ; Sarasin, A ; Menck, CFM. / Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair. In: Genetics and Molecular Biology. 2000 ; Vol. 23, No. 3. pp. 689-694.
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    abstract = "Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.",
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    Miyaji, EN, Johnson, RT, Downes, S, Eveno, E, Mezzina, M, Sarasin, A & Menck, CFM 2000, 'Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair', Genetics and Molecular Biology, vol. 23, no. 3, pp. 689-694.

    Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair. / Miyaji, EN; Johnson, RT; Downes, Stephen; Eveno, E; Mezzina, M; Sarasin, A; Menck, CFM.

    In: Genetics and Molecular Biology, Vol. 23, No. 3, 09.2000, p. 689-694.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair

    AU - Miyaji, EN

    AU - Johnson, RT

    AU - Downes, Stephen

    AU - Eveno, E

    AU - Mezzina, M

    AU - Sarasin, A

    AU - Menck, CFM

    PY - 2000/9

    Y1 - 2000/9

    N2 - Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.

    AB - Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.

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    SN - 1415-4757

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    Miyaji EN, Johnson RT, Downes S, Eveno E, Mezzina M, Sarasin A et al. Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair. Genetics and Molecular Biology. 2000 Sep;23(3):689-694.