Characterization of a fungal strain isolated from a polyphenol polluted site

V. Dritsa, F. Rigas, K. Natsis, R. Marchant

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A group of fungal strains were isolated from a polyphenol polluted soil, taken from an olive oil processing plant in Attica, Greece. The fungi were tested for their ability to decolorize a polyaromatic dye Poly R-478, which was used as a model compound to test their ligninolytic activities. The strain K1.1 decolorized efficiently the dye on agar plates and was further studied. PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA genes from the genomic DNA isolated from mycelium grown in liquid culture resulted in amplified fragments. Via BLASTN search, the length of a 773 base pairs was identified as the basidiomycetes Copri-nellus xanthothrix. The growth rates and the tolerance of the fungus were compared on solid media, containing four different concentrations of pentachlorophenol. Extracellular enzyme activities (lignin peroxidase, manganese peroxidase and laccase) were determined in defined liquid medium. The isolate expressed laccase and manganese peroxidase but not lignin peroxidase. The removal of the dye was also estimated in liquid medium. The fungus showed biosorption and biotransformation as removal mechanisms. (c) 2006 Elsevier Ltd. All rights reserved.
LanguageEnglish
Pages1741-1747
JournalBioresource Technology
Volume98
Issue number9
DOIs
Publication statusPublished - Jul 2007

Fingerprint

manganese peroxidase
lignin peroxidase
dyes
polyphenols
laccase
fungi
liquids
pentachlorophenol
biosorption
biotransformation
Basidiomycota
polluted soils
olive oil
Greece
internal transcribed spacers
mycelium
agar
ribosomal RNA
enzyme activity
genomics

Cite this

Dritsa, V. ; Rigas, F. ; Natsis, K. ; Marchant, R. / Characterization of a fungal strain isolated from a polyphenol polluted site. In: Bioresource Technology. 2007 ; Vol. 98, No. 9. pp. 1741-1747.
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Characterization of a fungal strain isolated from a polyphenol polluted site. / Dritsa, V.; Rigas, F.; Natsis, K.; Marchant, R.

In: Bioresource Technology, Vol. 98, No. 9, 07.2007, p. 1741-1747.

Research output: Contribution to journalArticle

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