Characterization and distribution of neuropeptide Y in the brain of a caecilian amphibian

Ted J. Ebersole; J. Michael Conlon; Frederick W. Goetz; Sunny K. Boyd

doi:10.1016/S0196-9781(01)00334-5

Characterization and distribution of neuropeptide Y in the brain of a caecilian amphibian

Ted J. Ebersole
, J. Michael Conlon
, Frederick W. Goetz
, Sunny K. Boyd

School of Biomedical Sciences

University of Notre Dame

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Neuropeptide Y (NPY) from the brain of an amphibian from the order Gymnophiona (the caecilian, Typhlonectes natans) was characterized. We cloned a 790 base pair cDNA encoding the caecilian NPY precursor. The open reading frame consisted of 291 bases, indicating an NPY precursor of 97 amino acids. Both deduced and isolated NPY primary structures were Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu¹⁰-Asp-Ala-Pro-Ala- Glu-Asp-Met-Ala-Lys-Tyr²⁰-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile- Asn-Leu³⁰-Ile-Thr-Arg-Gln-Arg-Tyr · NH2. In caecilian brain, we observed NPY immunoreactive cells within the medial pallium, basal forebrain, preoptic area, midbrain tegmentum and trigeminal nucleus. The prevalence of preoptic and hypothalamic terminal field staining supports the hypothesis that NPY controls pituitary function in this caecilian.

Original language	English
Pages (from-to)	325-334
Number of pages	10
Journal	Peptides
Volume	22
Issue number	3
DOIs	https://doi.org/10.1016/S0196-9781(01)00334-5
Publication status	Published (in print/issue) - 2001

Funding

We thank Drs. H. Vaudry and M. Tonon, University of Rouen, France for gifts of NPY antiserum (for immunocytochemistry) and synthetic frog NPY. Antiserum 8999 (for radioimmunoassay) was a generous gift from Dr. M. M. T. O’Hare, Queen’s University of Belfast, N. Ireland. We thank P.F. Nielsen, Novo Nordisk, Bagrvaerd, Denmark for mass spectrometry measurements and Cold Spring Harbor Laboratory for materials used to make a caecilian brain cDNA library. This work was supported by the National Science Foundation (IBN#98-06997 and IBN#95-14305).

Funders	Funder number
National Science Foundation	IBN#98-06997, 95-14305

Keywords

Gymnophiona
NPY gene
Pituitary
Reproduction
Typhlonectes natans

Access to Document

10.1016/S0196-9781(01)00334-5

Cite this

@article{0dcff8ba15e845d9a3aabe9d4a0d71f2,

title = "Characterization and distribution of neuropeptide Y in the brain of a caecilian amphibian",

abstract = "Neuropeptide Y (NPY) from the brain of an amphibian from the order Gymnophiona (the caecilian, Typhlonectes natans) was characterized. We cloned a 790 base pair cDNA encoding the caecilian NPY precursor. The open reading frame consisted of 291 bases, indicating an NPY precursor of 97 amino acids. Both deduced and isolated NPY primary structures were Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu10-Asp-Ala-Pro-Ala- Glu-Asp-Met-Ala-Lys-Tyr20-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile- Asn-Leu30-Ile-Thr-Arg-Gln-Arg-Tyr · NH2. In caecilian brain, we observed NPY immunoreactive cells within the medial pallium, basal forebrain, preoptic area, midbrain tegmentum and trigeminal nucleus. The prevalence of preoptic and hypothalamic terminal field staining supports the hypothesis that NPY controls pituitary function in this caecilian.",

keywords = "Gymnophiona, NPY gene, Pituitary, Reproduction, Typhlonectes natans",

author = "Ebersole, \{Ted J.\} and Conlon, \{J. Michael\} and Goetz, \{Frederick W.\} and Boyd, \{Sunny K.\}",

year = "2001",

doi = "10.1016/S0196-9781(01)00334-5",

language = "English",

volume = "22",

pages = "325--334",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Characterization and distribution of neuropeptide Y in the brain of a caecilian amphibian

AU - Ebersole, Ted J.

AU - Conlon, J. Michael

AU - Goetz, Frederick W.

AU - Boyd, Sunny K.

PY - 2001

Y1 - 2001

N2 - Neuropeptide Y (NPY) from the brain of an amphibian from the order Gymnophiona (the caecilian, Typhlonectes natans) was characterized. We cloned a 790 base pair cDNA encoding the caecilian NPY precursor. The open reading frame consisted of 291 bases, indicating an NPY precursor of 97 amino acids. Both deduced and isolated NPY primary structures were Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu10-Asp-Ala-Pro-Ala- Glu-Asp-Met-Ala-Lys-Tyr20-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile- Asn-Leu30-Ile-Thr-Arg-Gln-Arg-Tyr · NH2. In caecilian brain, we observed NPY immunoreactive cells within the medial pallium, basal forebrain, preoptic area, midbrain tegmentum and trigeminal nucleus. The prevalence of preoptic and hypothalamic terminal field staining supports the hypothesis that NPY controls pituitary function in this caecilian.

AB - Neuropeptide Y (NPY) from the brain of an amphibian from the order Gymnophiona (the caecilian, Typhlonectes natans) was characterized. We cloned a 790 base pair cDNA encoding the caecilian NPY precursor. The open reading frame consisted of 291 bases, indicating an NPY precursor of 97 amino acids. Both deduced and isolated NPY primary structures were Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu10-Asp-Ala-Pro-Ala- Glu-Asp-Met-Ala-Lys-Tyr20-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile- Asn-Leu30-Ile-Thr-Arg-Gln-Arg-Tyr · NH2. In caecilian brain, we observed NPY immunoreactive cells within the medial pallium, basal forebrain, preoptic area, midbrain tegmentum and trigeminal nucleus. The prevalence of preoptic and hypothalamic terminal field staining supports the hypothesis that NPY controls pituitary function in this caecilian.

KW - Gymnophiona

KW - NPY gene

KW - Pituitary

KW - Reproduction

KW - Typhlonectes natans

UR - https://www.scopus.com/pages/publications/0035088978

U2 - 10.1016/S0196-9781(01)00334-5

DO - 10.1016/S0196-9781(01)00334-5

M3 - Article

C2 - 11287086

AN - SCOPUS:0035088978

SN - 0196-9781

VL - 22

SP - 325

EP - 334

JO - Peptides

JF - Peptides

IS - 3

ER -

Characterization and distribution of neuropeptide Y in the brain of a caecilian amphibian

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this